Tag Archives: pubertal age

Aldehyde reductase activity and blood aldo-keto reductase spectrum in adolescents with neuroendocrine obesity

D. K. Kulieshova1, V. V. Davydov1, V. M. Shvets2

1Institute of Children and Adolescents Health Care,
National Academy of Medical Sciences of Ukraine, Kharkiv;
2Zaporozhye State Medical University, Ukraine;
e-mail: darya.kuleshova@gmail.com

Investigation of aldehyde-reductase activity and blood aldo-keto reductase spectrum has been performed in 13-15 and 16-18-years old adolescents with obesity to clear up the mechanisms of neuroendocrine obesity at the age of puberty. It has been established that basal aldehyde reductase activity and blood aldo-keto reductase spectrum of healthy adolescents in early puberty do not differ from those of healthy adolescents in late puberty. A decreased aldehyde reductase activity and some alterations in blood aldo-keto reductase spectrum have been observed in late puberty in adolescents with neuroendocrine obesity. In adolescents with obesity there have been registered some changes in blood aldo-keto reductase spectrum which are not accompanied by any alterations in its aldehyde reductase activity. The results obtained suggest that certain prerequisites are formed in late puberty to complicate the course of neuroendocrine obesity.

Oxidative stress and the enzyme system of aldehyde catabolism in the muscle mitochondria of immobilized pubertal rats

Amjad Hamdallah1, V. V. Davydov2, V. N. Shvets3

1V. N. Karazin Kharkiv National University, Ukraine;
2SU Institute of Children and Adolescent Health Care, National Academy
of Medical Science of Ukraine;
3Zaporizhzhia State Medical University, Ukraine;
e-mail: vaddavydov@mail.ru

The aim of the work is to find out peculiarities in manifestation of oxidative stress and to determine activity of enzymes, responsible for utilization of endogenous aldehydes in the mitochondrial fraction of the skeletal (femoral) muscle in pubertal rats during immobilization stress. Our study has shown that differently directed changes in the activity of mitochondrial aldehyde dehydrogenases and aldehyde reductases occur in the pubertal immobilized rats, that limits the catabolism effectiveness as regards carbonyl products of free radical oxidation in the muscle cells.  Corroboration of the effect under consideration is an increased level of protein free radical oxidation products in the mitochondria of the skeletal muscle. On the basis of the obtained data the authors draw a conclusion about an increased sensitivity of the skeletal muscle to the oxidative stress impact due to modulation in the state of enzyme system, responsible for utilization of endogenous aldehydes in the mitochondria.