Tag Archives: soybean mosaic virus
Screening of soybean plants for Rsv1 resistance gene and antioxidant enzyme isoforms under conditions of soybean mosaic virus infection
L. T. Mishchenko1*, O. О. Molodchenkova2*, A. A. Dunich1,
I. A. Mishchenko3, A. V. Dashchenko3, P. S. Tykhonov2,
I. I. Motsniy2, Ya. S. Fanin2
1Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”, Ukraine;
*e-mail: lmishchenko@ukr.net;
2Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation,
Laboratory of Plant Biochemistry, Odesa, Ukraine;
*e-mail: olgamolod@ukr.net;
3National University of Life and Environmental Sciences of Ukraine, Kyiv
Received: 11 May 2025; Revised: 20 August 2025;
Accepted: 30 January 2026; Available on-line: 23 February 2026
Soybean mosaic virus (SMV) infection is recognized as the most serious, long-standing problem in soybean producing areas in the world. The Rsv1 locus is a part of resistance genes family involved in plant defense mechanisms against pathogens. Rsv1 and antioxidant enzymes peroxidase and superoxide dismutase are known for their role in providing resistance to the soybean mosaic virus. This work aimed at screening soybean plants both SMV infected and healthy on the presence of Rsv1 locus and peroxidase and superoxide dismutase isozyme patterns. The presence of 3gG2 gene at the Rsv1 locus was detected with PCR in uninfected plants of 17 studied soybean varieties. The presence of 3gG2 gene was also revealed in SMV-infected plants of four varieties indicating that the gene was not expressed in these plants. electrophoresis in PAAG demonstrated that the spectrum of peroxidase and superoxide dismutase isoforms in soybean leaf tissues depends on genotype specificity and the presence/absence of the 3gG2 gene at the Rsv1 locus. The outcome of work will be useful for identification genotypes resistant to SMV and their implementation in soybean breeding programs in Ukraine.
The impact of soybean mosaic virus infection on biochemical composition of soybean seed
O. О. Molodchenkova1*, A. V. Dashchenko2, I. A. Mishchenko2,
A. A. Dunich3, I. I. Motsniy1, P. S. Tykhonov1, L. T. Mishchenko3
1Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation,
Laboratory of Plant Biochemistry, Odesa, Ukraine;
*e-mail: olgamolod@ukr.net;
2National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine;
3Taras Shevchenko National University of Kyiv,
ESC “Institute of Biology and Medicine”, Ukraine;
e-mail: lmishchenko@ukr.net
Received: 02 September 2024; Revised: 01 October 2024;
Accepted: 21 February 2025; Available on-line: 03 March 2025
It is known that infection caused by soybean mosaic virus (SMV) has a negative effect on soybean yield, productivity and seed quality. The main indicators of the nutritional value of soybean seed are the content of main storage proteins glycinin and β-conglycinin, as well as flavonoids, carbohydrates and fat. The goal of the research was to study the impact of SMV infection on the biochemical composition of the seeds of the soybean varieties created by classical breeding methods (‘Kano’, ‘Kuban’, ‘Kordoba’) and varieties of transgenic soybean (‘Grimo’ and ‘Monro’). DAS-ELISA and two step RT-PCR were used to identify SMV. Protein electrophoresis was carried out in 15% polyacrylamide gel containing 0.1% SDS at pH 8.3 according to the Laemmli method. We detected an increase in the activity of trypsin inhibitor and lectins and a decrease flavonoids content in the infected seeds of studied varieties in comparison with the healthy seeds. Seeds of all infected varieties showed a decrease in glycinin content and glycinin/β-conglycinin ratio, with the exception of ‘Monroe’ infected seeds in which both glycinin and β-conglycinin contents were increased or at the level of healthy seeds. We can distinguish the variety ‘Kano’ with a high content of flavonoids, varieties ‘Kuban’ and ‘Grimo’ with increased or maintained levels of healthy seed content of carbohydrates, fat and proteins under infection conditions. The differences in the relative content of the components of β-conglycinin and glycinin subunits in infected seeds depending on the soybean variety were revealed. The obtained results can be used for the selection of soybean varieties resistant to viral infection and with high seed quality.