Tag Archives: TBARS
Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment
T. M. Horid’ko1, H. V. Kosiakova1, A. G. Berdyshev1, O. F. Meged1,
O. V. Onopchenko1, V. M. Klimashevsky1, О. S. Tkachenko1, V. R. Bazylianska1,
V. O. Kholin2, K. O. Peschana2, S. A. Mykhalskiy2, N. M. Hula1
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: TanGoRi@ukr.net
The impairment of cognitive functions is the most studied medical and social problem nowadays. The aim of this study was to evaluate the protective effects of N-stearoylethanolamine (NSE) on memory state, blood and brain biochemical parameters in rats under scopolamine-induced cognitive impairment. The results of this study shown that NSE administration to rats per os (5 mg/kg, 5 days, during last 3 days NSE was administrated 20 min prior to scopolamine injection (1 mg/kg, once daily for 3 days, intraperitoneally)) prevented the development of memory impairment. In particular, NSE action was associated with the prevention of increase in acetylcholinesterase activity, changes in phospholipid, free and esterified cholesterol level in hippocampus and frontal cortex, and disruption in pro-/antioxidant balance in blood and studied brain sections. Considering the above mentioned biological effects, NSE is a promising drug candidate for integrative therapy of cognitive impairment of different profiles.
Antistress effects of N-stearoylethanolamine in rats with chronic social stress
T. M. Horid’ko1, H. V. Kosiakova1, A. G. Berdyshev1, O. F. Meged1,
E. A. Gudz1, O. V. Onopchenko1, V. S. Asmolkova1, V. M. Lozova2,
E. V. Tukalenko2, O. V. Bondarenko2, I. I. Tubalzeva2,
О. А. Kovalenko2, M. Y. Makarchuk2, N. M. Hula1
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: TanGoRi@ukr.net
The aim of this study was to evaluate the effects of N-stearoylethanolamine (NSE) on the number of biochemical parameters that are involved in the development of the stress response (corticosterone, catecholamines, testosterone, TBARS, nitric oxide, serotonin), memory state, exploratory activity and antinociceptive response in rats, using a model of chronic social stress. It has been demonstrated that administration of NSE (14 days , intragastrically, 50 mg/kg) after chronic social stress developed (stress was induced by daily agonistic interaction between animals), normalizes plasma content of corticosterone, testosterone, epinephrine, norepinephrine, dopamine, TBARS, nitrite-anion and increases serotonin level that leads to memory state improvement and increase in exploratory activity in rats. The tail-flick latency did not change, that is it remained at the level of stressed animals.