Tag Archives: ultrasound
Influence of variuos regimens ultrasaund on oxide-modified actomyosin superprecipitation reaction from skeletal muscle of rabbit
O. V. Shelyuk, N. Ye. Nurishchenko, K. O. Medynska
Taras Shevchenko Kyiv National University, Ukraine;
e-mail: shelyuk_olga@ukr.net
A comparative study of rabbit skeletal muscles oxide-modified actomyosin superprecipitation reaction in dependence on continuous and impulsive (2 ms) ultrasound regimens was studied. From the analyses of kinetic curves the effect of the value of superprecipitation (Аm – А0), time t1/2, required for achievement of half of its value was determined, and the normalized maximal rate of this reaction Vn was also calculated. It is shown that the use of continuous ultrasound to oxide-modified actomyosin was associated with a significant decrease of superprecipitation relative to controls. However, pulsed ultrasound caused a significant increase in superprecipitation value except for the values (Аm – А0) in the application of the intensity of 0.2 W/cm2. The oxide-modified actomyosin superprecipitation value under the effect of continuous and impulsive ultrasound at intensity 1 W/cm2 in relative to control and all other applied intensities decrease to the most extent. It is caused perhaps by thermal influence of ultrasound. In general, the data obtained give reason to assume that the effects of continuous and pulsed ultrasound on the reaction of oxide-modified proteins complex superpretsipitatsii identical.
ATPase activity of rabbit skeletal muscles actomyosin complex under ultrasound effect
K. O. Medynska, N. Ye. Nurishchenko, L. I. Pelyukh, O. V. Shelyuk
Taras Shevchenko Kyiv National University;
Educational and Scientific Centre “Institute of Biology”, Ukraine;
e-mail: medinkat@ukr.net
Influence of continuous and impulsive ultrasound 0.05; 0.2; 0.4; 0.7 and 1.0 W/cm2 on ATPase activity of rabbit skeletal muscle actomyosin has been investigated in this work. It has been shown that most changes of Mg2+,Ca2+-ATPase activity are observed under 0.2 and 0.4 W/cm2 continuous ultrasound. K+-ATPase activity is inhibited by continuous ultrasound of all intensities studied. Impulsive 2 and 10 ms ultrasound did not change the Mg2+,Ca2+-ATPase activity. While K+-activity is reliably changed only under impulsive 0.7 and 1.0 W/cm2 ultrasound that can be explained by the thermal effect. It has been determined, when studying the reconstructed actomyosin with sound troponin complex, that troponin complex is the most ultrasound sensitive constituent of actomyosin.