Ukr.Biochem.J. 2014; Volume 86, Issue 3, May-Jun, pp. 98-106

doi: http://dx.doi.org/10.15407/ubj86.03.098

Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome

17.06.2015   Uncategorized   No comments

Yu. I. Gubskyi1, T. A. Buhtiarova1, H. G. Goriushko1, N. V. Litvinova1,
G. I. Paramonova2, T. N. Kurapova1, O. N. Velychko1, L. P. Babenko1

1SI Institute of Pharmacology and Toxicology, National Academy of Medical Sciences of Ukraine, Kyiv;
2SI D.F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: iurigala@ukr.net

Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.

Keywords: , , , , ,

References:

  1. Gubsky IuI, Khobzei MK. Pharmacotherapy in palliative and hospice medicine. Clinical, pharmaceutical, medicolegal aspects. K.: Zdorov’ia, 2011. 352 p.
  2. Bobrov OE, Bryndikov LN, Kravchenko AV. Treatment of pain syndrome in oncology. Rovno: Kalligraf, 2003. 196 p.
  3. Approaching Death: Improving Care at the End of Life. Marilyn J. Field and Christine K. Cassel, Editors. Committee on Care at the End of Life, Institute of Medicine. 1997: P. 1–456. CrossRef
  4. Portenoy RK. Cancer pain. Epidemiology and syndromes. Cancer. 1989 Jun 1;63(11 Suppl):2298-307. PubMed, CrossRef
  5. Moulin DE. Pain in multiple sclerosis. Neurol Clin. 1989 May;7(2):321-31. PubMed
  6. Trynus F. P. Medical aspects of pain management and palliative care. Visnyk Farmakolohii i Farmatsii. 2007;(5):4–8.
  7. Bondar GA, Cherniy VI, Bondar BG., Yelsky VM, Kryukov MB. Chronic pain in cancer patients. Donetsk, 2010. 236 p.
  8. Palliative care to oncologic patients. Eds G. A. Novikov, V. I. Chissov. M., 2006. 192 p.
  9. Hubskyi YuI,  Buhtiarova TA,  Horiushko HH,  Litvinova NV,  Paramonova HI,  Babenko LP,  Kurapova TM,  Velychko OM. Studying of biochemical and structurally-dynamic parameters of erythrocytes membranes in the conditions of acute algetic syndrome and action of ketorolac and a-toxoferol acetate. Med Clin Chem. 2012;14(4(53)):5-11.
  10. Gubsky IuI, Bukhtiarova TA, Paramonova HI. Control of pain as a problem of clinical and molecular pharmacology. Biochemical and immunological responses in the development of pain syndrome. In the book: “Current issues of palliative and hospice care in Ukraine”.  Kyiv, 2012. P. 115-–120.
  11. Golovenko NYa. Physicochemical Pharmacology. Odessa: Astroprint, 2004. 720 p.
  12. Gubsky IuI, Stechenko OV, Goriushko HH. The study of molecular mechanisms of interaction of physiologically active compounds with biological structures under cell peroxide pathology.  In book: “Current issues of pharmaceutical and medical sciences and practices”.  Zaporizhia, ZSMU, 2006. P. 589–590.
  13. Gubsky  YuI,  Goryushko АG., Litvinova NV, Makovetsky VP, Kurapova TN,  Aphanasenko ОV, Velichko AN.  Antioxidant and membranotropic action of monochromanes and trimethylphenols derivatives in vitro.  Ukr Biokhim Zhurn. 2008 Nov-Dec;80(6):79–85. PubMed
  14. Hubskyi IuI, Bielenichev IF, Horiushko HH, Litvinova NV, Kovalenko SI, Babenko LP, Velychko OM. The study of antioxidant and membrane-acting properties of new S-containing quinazolone derivatives.  Pharmacol. Drug Toxicol. 2008;(1–3):80–86.
  15. Bukhtiarova T A. Experimental substantiation of the direction of investigation and study of new non-opioid analgesic in a series of nitrogen heterocycle derivatives: avtoref. dys. … d-ra med. nauk. Odesa, 1998. 35 p.
  16. Gubsky YuI, Trynus FP, Bukhtiarova TA. et al.  Zhurn Akad Med. Nauk Ukrainy. 1999;(1):24–28.
  17. Hubsky YuI, Horiushko AG, Bukhtiarova TA, Yadlovsky OYe. Membranotropic action of new nonopioid analgetic pirodazol.  Med. Khimiya. 2000;2(4):5–8.
  18. Preclinical drug researches.  Guidelines.  Edit. O. V. Stefanov. K, 2001. 527 p.
  19. Chuyan EN, Dzheldubaeva ER. Антиноцицептивное действие низкоинтенсивного электромагнитного излучения крайне высокой частоты при тонической боли. Sci. Notes Taurida Nat. V. I. Vernadsky Univ. 2005;18(57)(2):178-188. Series “Biology, Chemistry”.
  20. Laboratory Methods in the Clinic: A Handbook. Ed.  Menshikov V.V. M .: Medicine, 1987.  P. 119-120.
  21. Dobretsov GE Fluorescent Probes in the study of cells, membranes and lipoproteins. M.: Nauka, 1989. 274 p.
  22. Vladimirov YuV, Dobretsov GE. Fluorescent Probes in the study of biological membranes. M.: Nauka, 1980. 277 p.
  23.  Demchenko AP. Luminescence and dynamics of the protein structure. K.: Naukova dumka, 1988. 280 p.
  24. Otagiri M, Hardee GE, Perrin JH. Microcalorimetric investigations of pharmaceutical complexes II. Drug-albumin interactions. Biochem Pharmacol. 1978 May 1;27(9):1401-4. PubMed, CrossRef
  25. Biophysics. Ed. P.G. Kostyuk.  K.:Vyshcha shkola, 1988. 504 p.
  26. Petrenko YuM, Vladimirov YuA. A new property of aspirin and other salicylates. Their capacity for radical generation at the expense of  chelating-oxidizing action on iron cations. Eks. Klin. Farmakol. 1998;61(1):44-50.
  27. Trinus F, Bukhtiarova T, Yadlovsky O. Non-narcotic analgesics in the anesthesia. Visnyk Farmakolohii i Farmatsii. 2007;(5):13–19.
  28. Landau MA. Molecular mechanism of action of physiologically active compounds. Moscow: Nauka, 1981. 262 p.
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