Ukr.Biochem.J. 2016; Volume 88, Issue 3, May-Jun, pp. 5-17

doi: https://doi.org/10.15407/ubj88.03.005

Hydrolytic enzymes expressivity in different parts of the Rapana digestive system

V. A. Toptikov, V. N. Totsky, T. G. Alieksieieva, O. A. Kovtun

Odesa National Mechnуkov University, Ukraine;
e-mail: wat.22@mail.ru

The relevance of comprehensive studies of the Rapana vital functions is determined by its considerab­le negative impact on the ecosystem of the Black Sea. The aim of the work was to find out the polymorphism and activity of the main hydrolases in the different parts of the digestive system of Rapana. Hydrolases (proteases, amylases, esterases, lipases and phosphatases) in glandular structures of the Rapana digestive system were studied by electrophoresis. It was found that different sets of hydrolytic enzymes are functioning in certain parts of the Rapana digestive tract. The gland of Leiblein and hepatopancreas played the most important role in the digestion of food components. The salivary glands had the significant influence on proteolysis.

Keywords: , , , , , , ,


References:

  1. Drapkin EI. The new mollusk in the Black Sea. Priroda. 1953;(9):92-95. (In Russian).
  2. Chukhchin VD. Functional morphology of Rapana. K.: Naukova Dumka, 1970. 138 p. (In Russian).
  3. Zolotarev V. The Black Sea ecosystem changes related to the introduction of new mollusk species. Marine Ecology. 1996;17(1-3):227-236. CrossRef
  4. Mann R, Harding JM. Salinity tolerance of larval Rapana venosa: implications for dispersal and establishment of an invading predatory gastropod on the North American Atlantic coast. Biol Bull. 2003 Feb;204(1):96-103. PubMed, CrossRef
  5. Gaevskaya AV. Parasites, diseases and pests of mussels (Mytilus, Mytilidae). II. Mollusca. Sevastopol: EKOSI-Gidrofizika, 2006. 100 p.  (In Russian).
  6. Zaika V, Sergeeva N, Kolesnikova E. Alien species in bottom macrofauna of the Black Sea: their distribution and influence on benthic communities. Marine Ecolog J. 2010;ІХ(1):5-7. (In Russian).
  7. Chukhchin V. D. The ecology of gastropods of the Black Sea. K.: Naukova Dumka, 1984. 176 p. (In Russian).
  8. Kantor YuI.  Biological and Historical Mysteries of Rapa. Priroda. 2003;(5):32-34. (In Russian).
  9. Savini D, Occhipinti-Ambrogi A. Consumption rates and prey preference of the invasive gastropod Rapana venosa in the Northern Adriatic Sea. Helgol Mar Res. 2006 Feb 1;60(2):153-159. CrossRef
  10. Shadrin NV, Afanasyev TA. Nutrition and the distribution of Rapana venosa (Vallenciennes, 1846) in the waters of Opuksky Reserve (Eastern Crimea, the Black Sea). Marine Ecolog J. 2009;8(2):24. (In Russian).
  11. Snigirov S, Medinets V, Chichkin V, Sylantyev S. Rapa whelk controls demersal community structure off Zmiinyi Island, Black Sea. Aquatic Invasions. 2013;8(3):289-297. CrossRef
  12. International Council for the Exploration of the Sea. Alien Species Alert: Rapana Venosa (veined whelk). Edited by Roger Mann, Anna Occhipinti, and Juliana M. Harding. ICES Cooperative Research Report, 2004; 264: 14 p.
  13. The chemical composition of foods. Bk. 2: Reference table of amino acids, fatty acids, vitamins, macro- and micronutrients, organic acids and carbohydrates.  Ed.: Skurikhin I. M., Volgarev M.N. 2nd ed., Rev. and add. M.: Agropromizdat, 1987.  360 p. (In Russian).
  14. Chandler EA, McDowell JR, Graves JE. Genetically monomorphic invasive populations of the rapa whelk, Rapana venosa. Mol Ecol. 2008 Sep;17(18):4079-91. PubMed, CrossRef
  15. Zou S, Li Q, Kong L. Additional gene data and increased sampling give new insights into the phylogenetic relationships of Neogastropoda, within the caenogastropod phylogenetic framework. Mol Phylogenet Evol. 2011 Nov;61(2):425-35. PubMed, CrossRef
  16. Zou S, Li Q, Kong L. Multigene barcoding and phylogeny of geographically widespread muricids (Gastropoda: Neogastropoda) along the coast of China. Mar Biotechnol (NY). 2012 Feb;14(1):21-34. PubMed, CrossRef
  17. Studenikina EI, Pavlenko LN. Reserves and characteristics Rapana thomassiana in the north-eastern part of the Black Sea. Abstracts of the VI All-Russian Conference of commercial invertebrates. M.: VNIRO, 2002. P. 177-188. (In Russian).
  18. Mihailov VV, Litvinenko NM. The features of the reserves and distribution of Rapana thomasiana near the Crimean coast of the Kerch Strait. Proc. of Int. Workshop (Murmansk, 19-21 March 2003). Murmansk: Murmansk Marine Biological Institute), 2003. P. 54-56. (In Russian).
  19. Saenko EМ. Dynamics of biochemical parameters of the Rapana (Rapana thomasiana) tissues in different periods of its annual cycle. Probl Fisher. 2008;9(4(36)):788-796. (In Russian).
  20. Davis BJ. Disc elektrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci. 1964 Dec 28;121:404-27. PubMed, CrossRef
  21. Pena LB, Tomaro ML, Gallego SM. Effect of different metals on protease activity in sunflower cotyledons. Electronic J Biotechnol. 2006;9(3):258-262. CrossRef
  22. Manchenko GP. Handbook of detection of enzymes on electrophoretic gels.  CRC Press LLC, 2003. 592 p.
  23. Burstone MS. Enzyme histochemistry and its application in the study of neoplasms. New York–London: Academic Press, 1962. 621 p.
  24. Toptikov VA, Diachenko LF, Totsky VM. Estimation of enzyme multiple molecular forms spectra using the index of system diversity level. Tsitol Genet. 2010 Jan-Feb;44(1):46-53. (In Russian). PubMed, CrossRef
  25. Barnes RSK, Calow PP, Olive PJW, Golding DW. The Invertebrates: A New Synthesis. M.: Mir Publishers. 1992. 583 p. (In Russian).
  26. Ugolev AM. Natural technologies of biological systems. Leningrad: Nauka, 1987. 317 p.  (In Russian).
  27. Korn ED. Clearing factor, a heparin-activated lipoprotein lipase. I. Isolation and characterization of the enzyme from normal rat heart. J Biol Chem. 1955 Jul;215(1):1-14. PubMed
  28. Fielding CJ, Fielding PE. Mechanism of salt-mediated inhibition of lipoprotein lipase. J Lipid Res. 1976 May;17(3):248-56. PubMed

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