Ukr.Biochem.J. 2018; Volume 90, Issue 4, Jul-Aug, pp. 45-51

doi: https://doi.org/10.15407/ubj90.04.045

Gentamicin and magnesium chloride normalize cholinesterase and ATPase activities in rats acutely exposed to dichlorvos (DDVP) pesticide

18.06.2018   Uncategorized   No comments

B. S. Ajilore1, A. E. Adewuyi2, T. O. Oluwadairo2

1Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria;
e-mail: doctorajibam@yahoo.com;
2Department of Chemical Sciences, Faculty of Basic and Applied Sciences, College of Science, Engineering and Technology, Osun State University, Osogbo, Nigeria

This study investigated possible use of gentamicin and magnesium chloride as antidotes of dichlorvos pesticide poisoning. Thirty albino rats were randomly divided into 5 groups (n = 6). Group 1 served as negative control and received distilled water only. Group 2 served as positive control and was treated with 2.5 mg/kg body weight dichlorvos intraperitoneally. Group 3 was post-treated with 0.5 mg atropine following intraperitoneal 2.5 mg/kg dichlorvos while groups 4 and 5 rat were post-treated with 28 mg/kg intramuscular magnesium chloride and 5 mg/kg intramuscular gentamicin respectively following intraperitoneal 2.5 mg/kg dichlorvos. Plasma and red blood cell acetylcholinesterase activities were estimated. Total ATPase, Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase activities were estimated in the brain. Results showed significant (P < 0.05) increase in acetylcholinesterase activities in rats post treated with Atropine, MgCl2 and Gentamicin when compared with acetylcholinesterase activities in rats treated with dichlorvos only. There is significant (P < 0.05) increase in the activities of Ca2+,Mg2+-ATPases, Na+/K+-ATPase and total ATPase activities in the brain of rats post treated with atropine, magnesium chloride and gentamicin. Dichlorvos significantly (P < 0.05) reduced plasma and red blood cell cholinesterase activities, and brain ATPases activities. We concluded that dichlorvos toxicity inhibited cholinesterase, Na+/K+-ATPase and Ca2+,Mg2+-ATPases activities. Magnesium chloride and gentamicin on the other hand reduced effects of dichlorvos poisoning by promoting normal ATPase activities and inhibiting release of acetylcholine from cell. We proposed that both magnesium chloride and gentamicin can be co-administered in future as antidotes to patients with dichlorvos poisoning.

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References:

  1. Lotti M. Clinical toxicology ofanticholinesterase agents in humans. In:Krieger R, (ed). Handbook of pesticidetoxicology. Volume 2. Agents. 2nd ed. Academic Press; San Diego. 2001; pp. 1043–1085.
  2. Jeyaratnam J. Acute pesticide poisoning: a major global health problem. World Health Stat Q. 1990;43(3):139-44. PubMed
  3. Eddleston M, Buckley NA, Eyer P, Dawson AH. Management of acute organophosphorus pesticide poisoning. Lancet. 2008 Feb 16;371(9612):597-607. PubMed, PubMedCentral, CrossRef
  4. Pajoumand A, Shadnia S, Rezaie A, Abdi M, Abdollahi M. Benefits of magnesium sulfate in the management of acute human poisoning by organophosphorus insecticides. Hum Exp Toxicol. 2004 Dec;23(12):565-9. PubMed, CrossRef
  5. Shetab-Boushehri SV, Shetab-Boushehri SF, Abdollahi M. Possible role of Mg2+ ion in the reaction of organophosphate (dichlorvos) with serine. J Med Hypotheses Ideas. 2012; 6(1): 53-57.  CrossRef
  6. Paudyal BP. Organophosphorus poisoning. J Nepal Med Assoc. 2008 Oct-Dec;47(172):251-8. PubMed
  7. Eddleston M, Karalliedde L, Buckley N, Fernando R, Hutchinson G, Isbister G, Konradsen F, Murray D, Piola JC, Senanayake N, Sheriff R, Singh S, Siwach SB, Smit L. Pesticide poisoning in the developing world – a minimum pesticides list. Lancet. 2002 Oct 12;360(9340):1163-7. PubMed, CrossRef
  8. Ohkawa H. Stereoselectivity of organophosphorus insecticides. In: Insecticide mode of action. (Coats J.R. ed.). New York: Academic Press. 1982; p. 163-185.  CrossRef
  9. Nozdrenko DM, Miroshnychenko MS, Soroca VM, Korchinska LV, Zavodovskiy DO. The effect of chlorpyrifos upon ATPase activity of sarcoplasmic reticulum and biomechanics of skeleta l muscle contraction. Ukr Biochem J. 2016 Mar-Apr;88(2):82-8. PubMed, CrossRef
  10. Herschlag D, Jencks WP. The effect of divalent metal ions on the rate and transition-state structure of phosphoryl-transfer reactions. J Am Chem Soc. 1987; 109(15):4665-74.  CrossRef
  11. Herschlag D, Jencks WP. Catalysis of the hydrolysis of phosphorylated pyridines by Mg(OH)+: a possible model for enzymatic phosphoryl transfer. Biochemistry. 1990 May 29;29(21):5172-9. PubMed, CrossRef
  12. Neal MJ. Medical pharmacology at a glance. 3rd edition. Blackwell Science Ltd, London. 1997; pp 18-19.
  13. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959 May;82(1):70-7. PubMed, CrossRef
  14. Evans DJ Jr. Membrane adenosine triphosphatase of Escherichia coli: activation by calcium ion and inhibition by monovalent cations. J Bacteriol. 1969 Nov;100(2):914-22. PubMed, PubMedCentral
  15. Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem. 1925; 66: 375-400.
  16. Bonting SL. Sodium-potassium activated adenosine triphosphatase and cation transport. In: Membrane and ion transport, Eds by Bittar, E.E. Wiley Inter Science, London. 1970; p257-263.
  17. Hjertén S, Pan H. Purification and characterization of two forms of a low-affinity Ca2+-ATPase from erythrocyte membranes. Biochim Biophys Acta. 1983 Feb;728(2):281-8.PubMed, CrossRef
  18. Ohnishi T, Suzuki T, Suzuki Y, Ozawa K. A comparative study of plasma membrane Mg2+ -ATPase activities in normal, regenerating and malignant cells. Biochim Biophys Acta. 1982 Jan 4;684(1):67-74. PubMed, CrossRef
  19. Mansour SA. Pesticide exposure–Egyptian scene. Toxicology. 2004 May 20;198(1-3):91-115. PubMed, CrossRef
  20. Gwary OM, Hati SS, Dimari GA, Ogugbuaja VO. Pesticide Residues in Bean Samples from Northeastern Nigeria. ARPN J Sci Technol. 2012; 2(2): 79-84.
  21. Yadwad VB, Kallapur VL, Basalingappa S. Inhibition of gill Na+ K(+)-ATPase activity in dragonfly larva, Pantala flavesens, by endosulfan. Bull Environ Contam Toxicol. 1990 Apr;44(4):585-9. PubMed, CrossRef
  22. Guyton AC, Hall JE. Textbook of medical physiology. 11th edition. Elsevier Inc. Philadelphia, PA, USA 2006.
  23. Carafoli E, Stauffer T. The plasma membrane calcium pump: functional domains, regulation of the activity, and tissue specificity of isoform expression. J Neurobiol. 1994 Mar;25(3):312-24. PubMed, CrossRef
  24. Barber D, Hunt J, Ehrich M. Inhibition of calcium-stimulated ATPase in the hen brain P2 synaptosomal fraction by organophosphorus esters: relevance to delayed neuropathy. J Toxicol Environ Health A. 2001 May 25;63(2):101-13. PubMed, CrossRef
  25. El-Fawal HA, Correll L, Gay L, Ehrich M. Protease activity in brain, nerve, and muscle of hens given neuropathy-inducing organophosphates and a calcium channel blocker. Toxicol Appl Pharmacol. 1990 Mar 15;103(1):133-42. PubMed, CrossRef
  26. Cowan JA. Structural and catalytic chemistry of magnesium-dependent enzymes. Biometals. 2002 Sep;15(3):225-35. PubMed, CrossRef
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