Ukr.Biochem.J. 2020; Volume 92, Issue 5, Sep-Oct, pp. 41-49


Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals

A. K. Gulevskyy*, Yu. S. Akhatova, A. Yu. Nikolchenko

Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkiv;

Received: 07 July 2019; Accepted: 25 June 2020

The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was studied. Cryoprotective agents within the range of concentrations which provide a cryoprotective effect were found to inhibit significantly protein synthesis in cell-free systems under investigation. The most effective inhibition was exerted by polymeric cryoprotective agent PEG-400. Cryoprotective agents more efficiently  inhibited protein synthesis at a cell level as compared with that in cell-free system that was likely associated with their effect on amino acids transport system. An inhibitory effect of cryoprotective agents on the protein synthesizing apparatus of cells was determined to be Mg2+-dependent and reversible.

Keywords: , , , , ,


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