Ukr.Biochem.J. 2021; Volume 93, Issue 6, Nov-Dec, pp. 76-86

doi: https://doi.org/10.15407/ubj93.06.076

Properties of alpha-L-fucosidase for serum of patients with hepatocellular cancer and cytotoxicity on some cancer cell lines

16.12.2021   Uncategorized   No comments

Z. M. A. A. Hamodat

University of Mosul, College of Science, Chemistry Department, Mosul – Iraq;
e-mail: zahraahamodat@uomosul.edu.iq

Received: 07 July 2021; Accepted: 12 November 2021

Alpha-L-fucosidase (FUCA) degrades many fucosylated glycans and has long been recognized as a tumor marker associated with the early detection of some cancers. This study aimed to purify and characterize alpha-L-fucosidase from the serum of patients with hepatocellular cancer and estimate its toxic effect against hepatocellular carcinoma HepG2, prostate cancer PC3 cell lines and the standard hepatocyte WRL-68 cell line. SDS-Page Electrophoresis technique was used to determine the purity of the purified alpha-L fucosidase and estimate its molecular weight. Three purification steps were used for FUCA purification: precipitation with 65% ammonium sulfate saturation, DEAE-cellulose ion exchange, Sephadex G-75 gel filtration. The procedure resulted in 54% recovery of the enzyme with 27.5-fold purification and 14 U/mg specific activity. It was demonstrated that FUCA purified from the serum of HCC patients showed a more toxic effect on HepG2 cells (IC50 of 65.74 µg/ml) than on PC3 prostate cancer cells (IC50 of 111.5 g/ml) and less toxic effect against standard hepatocyte WRL-68cells (IC50 of 214.5 µg/ml). We can conclude that the inhibitory effect of the purified FUCA on hepatocellular carcinoma is more than its effect on prostate cancer cells. Also, the purified FUCA may be used in studies on anticancer drug development in liver cancer.

Keywords: , , , ,

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