Tag Archives: adolescents

HIF-2α level in adolescents with chronic inflammatory pathology of the upper gastrointestinal tract

04.12.2024   Uncategorized   No comments

N. S. Shevchenko1,2, N. V. Krutenko1*, L. L. Sukhova2,
O. M. Tsiura1, H. O. Shlieienkova1, K. V. Voloshyn1

1V. N. Karazin Kharkiv National University, Kharkiv, Ukraine;
2State Institution “Institute for Children and Adolescents Health Care
at the National Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine;
*e-mail: n.v.krutenko@karazin.ua

Received: 08 September 2024; Revised: 08 November 2024;
Accepted: 21 November 2024; Available on-line: 17 December 2024

Сhronic inflammatory diseases of the gastrointestinal tract are among the most common pathological conditions in adolescents. A significant role in the pathogenesis of gastrointestinal tract diseases is given to the functioning of the oxygen sensor system, the main mediator of which is hypoxia-inducible factor 2 (HIF-2α). Therefore, the purpose of the study was to determine the level of HIF-2α in the plasma of patients with chronic inflammatory pathology of the gastrointestinal tract, taking into account the endoscopic picture of the lesion, localization of the pathological process, age and gender of the patient. The study involved 70 adolescents aged 8-18 years with chronic gastroduodenitis (CGD), gastroesophageal reflux disease (GERD), gastric or duodenal ulcer and 25 peers who had been classified as healthy. The plasma concentration of HIF-2α was measured by a sandwich-linked ELISA. Helicobacter pylori infection was determined by urease test or by ELISA. According to the data obtained, the HIF-2α plasma level was higher in patients with chronic inflammatory gastrointestinal disease compared to the control group. In boys with chronic gastroduodenal disease, the level of HIF-2α was higher than in girls. CGD and GERD were characterized by a higher HIF-2α level in plasma than gastric and duodenal ulcers. The HIF-2α level did not depend on the age of the patients or the presence of Helicobacter pylori infection.

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The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents

18.05.2021   Uncategorized   No comments

H. A. Pavlyshyn, A.-M. A. Shulhai*

Department of Pediatrics № 2, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: shulhai_aa@tdmu.edu.ua

Received: 29 January 2021; Accepted: 23 April 2021

An increase in the prevalence of overweight and obese adolescents is often combined with a low level of vitamin D that may be associated with lipid and carbohydrate metabolism disorders, which underlie the development of arterial hypertension and cardiometabolic changes. This work aimed to determine the association between vitamin D status and the main parameters of lipid and carbohydrate metabolism, and anthropometric measurements. A total of 196 adolescents (129 boys and 67 girls) from the Ternopil region were examined. Their mean age was 15.5 ± 2.3 years. Based on the body mass index (BMI): 60 adolescents had normal body weight, 60 were overweight, and 76 were obese. Anthropometric measurements and general examinations were performed. In blood serum 25(OH)D levels and the main parameters of lipid and carbohydrate metabolism were determined. Low levels of 25(OH)D were found. Prevalence of vitamin D deficiency among adolescents with BMI up to the 85th percentile was 56.7%, with BMI in the 85-97th percentiles was 70.0% and with BMI over the 97th percentile was 77.6%. Serum 25(OH)D levels in overweight and obese adolescents had a significant correlation with BMI (r = -0.427, P < 0.001), waist circumference (r = -0.462, P < 0.001), high-density lipoprotein cholesterol (HDL-C, r = 0.214, P = 0.023), low-density lipoprotein cholesterol (LDL-C, r = -0.226, P = 0.011), atherogenic index (r = -0.284, P = 0.001), insulin level (r = -0.483, P < 0.001), and HOMA-IR index (r = -0.454, P < 0.001). We concluded that the development of cardiometabolic disorders in overweight and obese adolescents is associated with vitamin D status.

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Indicators of lipid metabolism disorders in the blood serum of adolescents with metabolic syndrome

17.12.2020   Uncategorized   No comments

L. A. Strashok1,2, O. V. Buznytska1,2*, О. М. Meshkova3

1Kharkіv Medical Academy of Postgraduate Education, Ukraine;
2V.N. Karazin Kharkіv National University, Ukraine;
3Bogomolets National Medical University, Kyiv, Ukraine;
*e-mail: ebuznickaa@ukr.net; elena.buznytska@gmail.com

Received: 16 April 2018; Accepted:13 November 2020

Atherogenic dyslipidemia is one of the main and early indication of adolescentrs with metabolic syndrome. Because there is not enough information about the nature of dyslipidemia in adolescents and the possibilities of its correction and prevention, the study of this problem is relevant. The goal of the study was to estimate the indexes of lipid metabolism in blood serum of adolescents with metabolic syndrome. We examined 200 obese patients aged 14–18 years, who were divided into two groups with and without metabolic syndrome. The control group consisted of 30 adolescents of similar age with normal body weight. The lipid profile of blood was studied by standardized methods using Cormay Multi semi-automatic photometer­. The level of β-lipoproteins in blood serum was determined by the turbidimetric method. Elevated levels of triglycerides, low and very low density lipoprotein cholesterol, β-lipoproteins and a tendency to decreased high density lipoprotein level cholesterol were found in the  blood serum of adolescents with metabolic syndrome  as the signs of atherogenic dyslipidemia. Reliable correlations both between the indexes of lipid profile and between atherogenic dyslipidemia indicators and anthropometric indexes were established, indicating an increased risk of lipid metabolic disturbances in individuals with abdominal obesity.

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Diagnostic significance of biochemical indicators of liver fibrogenesis in adolescents with obesity

22.01.2019   Uncategorized   No comments

O. V. Buznytska

Kharkіv Medical Academy of Postgraduate Education,
V. N. Karazin Kharkіv National University, Ukraine
e-mail: ebuznickaa@ukr.net; missbuzelena@gmail.com

Received: 27 September 2018; Accepted: 13 December 2018

Non-alcoholic fatty liver disease occurs in most obese people, the main pathway of which is the process of fibrogenesis. The aim of this work was to determine the potential biomarkers for early diagnosis of liver fibrogenesis in adolescents with obesity. The levels of liver fibrosis markers, such as fibronectin, collagen type IV, N-terminal propeptides and C-terminal telopeptides of type I collagen, were assessed with the use of IFA method in serum of 226 patients with obesity aged 8-18 years. A significant increase in levels of type IV collagen and fibronectin was observed in children with obesity (P < 0.05). As diagnostic criteria for fibrogenesis and fibrolysis, the levels of N-terminal propeptides and C-terminal telopeptides of type I collagen, respectively, were determined. The serum level of N-terminal propeptides of type I collagen significantly exceeds the normal values in all children with obesity compared to the control group (P < 0.05). The biochemical markers (type IV collagen, fibronectin, N-terminal propeptides and C-terminal telopeptides of type I collagen) were proven to have high diagnostic informative value in the early diagnosis of liver fibrogenesis in obese adolescents. It was shown that the signs of fibrosis in non-alcoholic fatty liver disease already occur at the stage of steatosis.

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