Tag Archives: antibody

Levels of serum antibodies to enterobacterial lipopolysaccharides and their relationship with concentration of C-reactive protein in diabetes mellitus patients

A. I. Gordienko

S. I. Georgievsky Crimea state medical university, Simferopol;
e-mail: uu4jey@csmu.strace.net

We examined patients with type 1 (DM 1) and type 2 (DM 2) diabetes mellitus. The concentration of C-reactive protein (CRP) in the blood and levels of serum antibodies to different classes of enterobacterial lipopolysaccharides (LPS) were determined by ELISA. Using cluster analysis it was shown that in 40.8% DM-1 patients the increased concentration of CRP is associated with a decrease in the levels of serum anti-LPS-IgA, anti-LPS-IgM and anti-LPS-IgG. In 56.7% of DM-2 patients with increased concentration of CRP levels of serum anti-LPS-IgA and anti-LPS-IgM were not significantly different from the normal values, but the levels of serum anti-LPS-IgG were significantly increased. Activation of inflammation and increase of concentration of the CRP in the blood of DM-2 patients is accompanied by a significant increase in the levels of serum anti-LPS-A and anti-LPS-G, as well as the tendency to reduce the levels of anti-LPS-IgM. The results of this study suggest an association between low intensity inflammation and immune response to enterobacterial LPS  in type 1 and 2 diabetes mellitus.

Idiotype-antiidiotypic interaction of antibodies in the bloodstream

S. A. Bobrovnik

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
е-mail: s-bobrov@bk.ru

A problem of “prozone” formation in agglutination of corpuscular antibodies by bivalent antibodies­ was considered. Using a new coordinate system, which was proposed by us earlier, we analyzed theoretical and experimental curves that describe relations between bivalent antibody concentration and some blocking factors. It was shown that occupation of antibody paratopes by a blocking­ factor or by antiidiotypic antibodies can induce “prozone” formation. It was also demonstrated that experimental titration curves for a mixture of antibodies and corresponding antigens coincide with theoretical curves that were calculated according to our theory. Our data also demonstrate that major part of serum antibodies are blocked by antiidiotypic antibodies when maximum of antibody formation is over and antibody titers come down.