Tag Archives: exometabolites
Exometabolites of streptomycetes isolated from the Odesa bay exhibit a toxic effect against human cancer cell lines
V. O. Ivanytsia1, T. V. Gudzenko1*, O. H. Gorshkova2, G. V. Lisyutin2,
M. D. Shtenikov2, N. V. Korotaeva1, K. S. Potapenko1,
I. V. Strashnova2, T. V. Ivanytsia1
1Department of Microbiology, Virology and Biotechnology,
Odesa National I. I. Mechnikov University, Odesa, Ukraine;
2Scientific Center of Marine Biology and Biotechnology,
Odesa National I. I. Mechnikov University, Odesa, Ukraine;
*e-mail: tgudzenko@ukr.net
Received: 28 August 2023; Revised: 04 October 2023;
Accepted: 01 December 2023; Available on-line: 18 December 2023
The aim of the work was to determine the cytotoxic activity of marine actinobacteria exometabolites on the human larynx adenocarcinoma Hep-2 and rhabdomyosarcoma RD cell lines. Exometabolites of 6 strains of Streptomyces sp. were extracted with ethyl acetate and their composition was determined with UltiMate™ 3000 BioRS UPLC System coupled to mass spectrometer. Cytotoxic activity of exometabolites against cancer cells was determined after 24 hours of incubation by microscopic examination of cell morphological changes and the degree of cell monolayer degeneration. Exometabolites from Streptomyces sp. Myt7b strain showed the highest cytotoxic activity and at concentration of 500.0 μg/ml caused up to 90.0% death of RD and Hep-2 cells. The presence of 53 identified compounds with potential cytotoxic activity, including enterocin, 6-prenyltryptophol, medelamine A and turbinaric acid was detected in Streptomyces sp. Myt7b exometabolome. It is concluded that this strain is a promising producer of compounds with anticancer activity.
Influence of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 strain exometabolites on phenylalanine ammonia-lyase activity in winter wheat seedlings
I. V. Dragovoz, Yu. V. Korzh, N. O. Leonova,
V. M. Iliash, L. V. Аvdeeva
Zabolotny Institute of Microbiology and Virology,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: igordragovoz@ukr.net
Influence of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 strain exometabolites on phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) activity in winter wheat seedlings has been studied. A significant increase of PAL activity at 4-6 hours after treatment of plant roots with Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 strain exometabolites and in case of leaves infection with Bipolaris sorokiniana plant pathogen has been shown. It was established that PAL activity changed along with a decrease of the infected surface area of the leaves evidenced for the induction of response in winter wheat seedlings induced by IMV B-7404 strain exometabolites. It was concluded that the studied exponents could be used as model systems in the research of phytoimmunity induction mechanisms.