Tag Archives: glutathione dependent enzymes

The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4)

N. O. Salyha

Institute of Animal Biology NAAS of Ukraine, Lviv;
е-mail: ynosyt@yahoo.com

Received: 30 March 2023; Revised: 19 May 2023;
Accepted: 05 June 2023; Available on-line: 20 June 2023

A toxic organic substance CCl4 is a well known model compound for studying detoxification function of the liver and developing oxidative stress. The goal of the study was to estimate the effect of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) administration on rat blood parameters upon the toxic effects of CCl4. Experimental male Wistar rats were injected intraperitoneally with CCl4, the rats of CCl4/L‑Glu group were additionally injected with L-Glu (750 mg/kg), of CCl4/NAC group – with NAC (150 mg/kg), of CCl4/L-Glu/NAC group – with L-Glu (750 mg/kg) and NAC (150 mg/kg). The duration of the experiment was 24 h. Increased level of lipid peroxides, TBARS, triacylglycerols, cholesterol and decreased glutathione peroxidase, glutathione reductase, glutathione-S-transferase activity and GSH content were observed in the blood of the CCl4 treated animals compared to the control untreated group. When animals were additionally injected with L-Glu or L-Glu/NAC, the most of the studied indicators were shown to be close to the control level These results suggest that the mentioned above aminoacids attenuated CCl4-induced oxidative stress in the blood of rats.