Ukr.Biochem.J. 2023; Volume 95, Issue 2, Mar-Apr, pp. 68-74


The effect of L-glutamic acid and N-acetylcysteine administration on biochemical blood parameters in rats treated with CCl(4)

N. O. Salyha

Institute of Animal Biology NAAS of Ukraine, Lviv;

Received: 30 March 2023; Revised: 19 May 2023;
Accepted: 05 June 2023; Available on-line: 20 June 2023

A toxic organic substance CCl4 is a well known model compound for studying detoxification function of the liver and developing oxidative stress. The goal of the study was to estimate the effect of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) administration on rat blood parameters upon the toxic effects of CCl4. Experimental male Wistar rats were injected intraperitoneally with CCl4, the rats of CCl4/L‑Glu group were additionally injected with L-Glu (750 mg/kg), of CCl4/NAC group – with NAC (150 mg/kg), of CCl4/L-Glu/NAC group – with L-Glu (750 mg/kg) and NAC (150 mg/kg). The duration of the experiment was 24 h. Increased level of lipid peroxides, TBARS, triacylglycerols, cholesterol and decreased glutathione peroxidase, glutathione reductase, glutathione-S-transferase activity and GSH content were observed in the blood of the CCl4 treated animals compared to the control untreated group. When animals were additionally injected with L-Glu or L-Glu/NAC, the most of the studied indicators were shown to be close to the control level These results suggest that the mentioned above aminoacids attenuated CCl4-induced oxidative stress in the blood of rats.

Keywords: , , , , ,


  1. Ernst L, Zieglowski L, Schulz M, Moss M, Meyer M, Weiskirchen R, Palme R, Hamann M, Talbot SR, Tolba RH. Severity assessment in mice subjected to carbon tetrachloride. Sci Rep. 2020;10(1):15790. PubMed, PubMedCentral, CrossRef
  2. Li R, Zhang P, Li C, Yang W, Yin Y, Tao K. Tert-butylhydroquinone mitigates Carbon Tetrachloride induced Hepatic Injury in mice. Int J Med Sci. 2020;17(14):2095-2103. PubMed, PubMedCentral, CrossRef
  3. Scholten D, Trebicka J, Liedtke C, Weiskirchen R. The carbon tetrachloride model in mice. Lab Anim. 2015;49(1 Suppl):4-11. PubMed, CrossRef
  4. Hamed H, Gargouri M, Bellassoued K, Ghannoudi Z, Elfeki A, Gargouri A. Cardiopreventive effects of camel milk against carbon tetrachloride induced oxidative stress, biochemical and histological alterations in mice. Arch Physiol Biochem. 2018;124(3):253-260. PubMed, CrossRef
  5. Pergel A, Tümkaya L, Çolakoğlu MK, Demiral G, Kalcan S, Özdemir A, Mercantepe T, Yilmaz A. Effects of infliximab against carbon tetrachloride-induced intestinal injury via lipid peroxidation and apoptosis. Hum Exp Toxicol. 2019;38(11):1275-1282. PubMed, CrossRef
  6. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-126. PubMed, PubMedCentral, CrossRef
  7. Aruoma OI. Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol. 1994;32(7):671-683. PubMed, CrossRef
  8. Mutus B. The catalytic mechanism for NO production by the mitochondrial enzyme, sulfite oxidase. Biochem J. 2019;476(13):1955-1956. PubMed, PubMedCentral, CrossRef
  9. Cynober L. Metabolism of Dietary Glutamate in Adults. Ann Nutr Metab. 2018;73(Suppl 5):5-14. PubMed, CrossRef
  10. Salyha NO. L-glutamic acid effect on changes in biochemical parameters of rats intoxicated by carbon tetrachloride. Biol Tvarin. 2021;23(1):18-22. CrossRef
  11. Michlin M, Argaev-Frenkel L, Weinstein-Fudim L, Ornoy A, Rosenzweig T. Maternal N-Acetyl Cysteine Intake Improved Glucose Tolerance in Obese Mice Offspring. Int J Mol Sci. 2020;21(6):1981. PubMed, PubMedCentral, CrossRef
  12. Mahmoud SM, Abdel Moneim AE, Qayed MM, El-Yamany NA. Potential role of N-acetylcysteine on chlorpyrifos-induced neurotoxicity in rats. Environ Sci Pollut Res Int. 2019;26(20):20731-20741. PubMed, CrossRef
  13. Liu Y, Yao W, Xu J, Qiu Y, Cao F, Li S, Yang S, Yang H, Wu Z, Hou Y. The anti-inflammatory effects of acetaminophen and N-acetylcysteine through suppression of the NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes. Innate Immun. 2015;21(6):587-597. PubMed, CrossRef
  14. Lee SI, Kang KS. N-acetylcysteine modulates lipopolysaccharide-induced intestinal dysfunction. Sci Rep. 2019;9(1):1004. PubMed, PubMedCentral, CrossRef
  15. Salyha NO. Activity of the glutathione system of antioxidant defense in rats under the action of L-glutamic acid. Ukr Biokhim Zhurn. 2013;85(4):40-47. (In Ukrainian). PubMed, CrossRef
  16. Rosalovsky VP, Grabovska SV, Salyha YuT. Changes in glutathione system and lipid peroxidation in rat blood during the first hour after chlorpyrifos exposure. Ukr Biochem J. 2015;87(5):124-132. PubMed, CrossRef
  17. Li R, Huang C, Ho JCH, Leung CCT, Kong RYC, Li Y, Liang X, Lai KP, Tse WKF. The use of glutathione to reduce oxidative stress status and its potential for modifying the extracellular matrix organization in cleft lip. Free Radic Biol Med. 2021;164:130-138. PubMed, CrossRef

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