Tag Archives: hybridomas

Obtaining and study of properties of new monoclonal antibodies against human IgE

O. Yu. Galkin1,2, A. A. Savchenko1, K. I. Nikitina1, O. M. Dugan1

1National Technical University of Ukraine “Kyiv Polytechnic Institute”;
2Hema Ltd., Kyiv, Ukraine;
e-mail: alexfbt@mail.ru

The original set of 12 clones of hybridomas, producers of monoclonal antibodies (mAbs) against human IgЕ, has been obtained. The study of biological properties of antibodies has been conducted: their specificity, affinity constant and titer in a cultural medium have been established. The obtained mAbs are directed to the two epitope regions on IgE molecule. The first group of mAbs relates to epitope region, represented by three epitopes (two of them overlap and one that does not overlap with others). The second epitope region is represented by only one epitope.