S. O. Kosterin¹*, Т. О. Veklich¹, O. І. Kalchenko²,
A. I. Vovk³*, R. V. Rodik², О. А. Shkrabak¹

¹Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: kinet@biochem.kiev.ua;
²Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: manli@ioch.kiev.ua;
³V. P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry,
National Academy of Sciences of Ukraine, Kyiv;
*e-mail: vovk@bpci.kiev.ua

Received: 08 February 2024; Revised: 27 March 2024;
Accepted: 31 May 2024; Available on-line: 17 June 2024

The kinetic model of calix[4]arene-induced ATP hydrolysis was elaborated. It is assumed that calix[4]­arene С-107 molecules form a complex with nucleoside triphosphate, ensuring the release of inorganic phosphate Р_і, and then switch into an inactive state. Inactive calix[4]arene molecules are no longer able to form a complex with ATP and, accordingly, to provide hydrolysis of nucleoside triphosphate. In the author’s experimental studies, it was possible to explain the kinetic properties of the reaction, namely: the insignificant output of the reaction end product P_i; the quantitative regularities of the plateau (time-wise) accumulation of the reaction product when the concentration of calix[4]arene C-107 or ATP changes; the reciprocal dependence of the ATP half conversion on its concentration; the correspondence of the initial reaction rate dependence on the calix[4]arene and ATP concentration to the Michaelis-Menten equation. The final decision regarding the molecular mechanism of calix[4]arene-induced ATP hydrolysis requires further experimental and theoretical studies.