Tag Archives: leukocytes
Mitochondrial DNA copy number in leukocytes of patients with type 2 diabetes mellitus and chronic kidney disease
Y. A. Saienko1,2*, D. S. Krasnenkov1, K. K. Midlovets1, V. V. Korcheva1,
Y. E. Rebrova1,3, D. D. Yepishyna1, B. M. Mankovsky1
1SI “D.F. Chebotarev Institute of Gerontology, National Academy
of Medical Sciences of Ukraine”, Kyiv;
2SI “Center for Cardiology and Cardiac Surgery
of the Ministry of Health of Ukraine”, Kyiv
3P.L. Shupyk National University of Health Care of Ukraine, Kyiv;
*e-mail: ysaenko1981@gmail.com
Received: 04 April 2025; Revised: 23 April 2025;
Accepted: 25 April 2025; Available on-line: 12 May 2025
Chronic kidney disease (CKD) as one of the most common complications of type 2 diabetes mellitus (T2DM) significantly increases the risk of cardiovascular disease and mortality. Mitochondrial dysfunction, in particular a reduction in mitochondrial DNA copy number (mtDNA-CN), plays an important role in the development of diabetic complications, including nephropathy. The aim of this study was to determine the mtDNA-CN in peripheral blood leukocytes of patients with T2DM depending on the presence of CKD. A total of 109 individuals were examined, including 20 healthy controls and 89 patients with T2DM divided into groups based on the presence or absence of CKD. The mtDNA-CN in leukocytes was determined using quantitative real-time PCR. Biochemical markers of T2DM and CKD were evaluated, non-parametric tests and correlation analysis were performed. No statistically significant differences in mtDNA-CN level were observed between patients with T2DM and CKD, patients with T2DM without CKD, and the control group (P > 0.05). No associations between mtDNA-CN and kidney function parameters were identified. The absence of mtDNA-CN alterations is assumed to contribute to the relatively satisfactory glycemic control in diabetic groups.
High level of RNA HAS2-AS1 in the buffy coat of a patient blood sample is a more informative prognostic marker of COVID-19 clinical course compared to the level of hyaluronic acid in plasma
E. Dubrovskyi*, T. Drevytska, V. Dosenko, D. Stroy
Department of General and Molecular Pathophysiology,
Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: udjin1785@gmail.com
Received: 21 March 2024; Revised: 13 May 2024;
Accepted: 25 July 2024; Available on-line: 04 September 2024
Numerous studies have confirmed the association of COVID-19 clinical course with the blood levels of hyaluronic acid and long non-coding RNA HAS2-AS1 as a positive regulator of hyaluronan synthase. We aimed to estimate both the level of hyaluronic acid in plasma and the level of RNA HAS2-AS1 in leukocytes (buffy coat) from the same blood sample at the time of patient admission to the hospital and to analyze the specificity of these indicators as biomarkers of COVID-19 severity. The study involved 78 patients with confirmed COVID-19 who underwent treatment between 2020 and 2021 at the Kyiv City Clinical Hospital N 4. The patients were divided into three groups based on the severity of the disease and classified as mild (n = 12), moderate (n = 36), and severe (n = 30). The level of hyaluronic acid in plasma was determined using the Hyaluronic Acid ELISA kit “Abcam” (USA). The buffy coat was isolated by centrifugation of the blood stabilized with EDTA-K and further aspiration of the leukocyte “cloud”. The expression level of HAS2-AS1 in buffy coat leukocytes was estimated using reverse transcription and real-time PCR. According to the obtained data, the level of hyaluronic acid in the plasma of patients with moderate and severe illness was 1.5 and 2.2 times higher compared to the mild illness group, respectively. Meanwhile, the level of RNA HAS2-AS1 in blood lymphocytes (buffy coat) of patient with moderate and severe illness was increased by 7.7 and 22.6 times compared to patients with mild illness. The results of our study demonstrate that, unlike the level of hyaluronic acid in plasma, the level of HAS2-AS1 in a buffy coat is a more reliable prognostic criterion for severe COVID-19 and allows distinguishing patients with varying clinical severity during hospitalization.
Influence of poly(ADP-ribose)polymerase inhibitors on some parameters of oxidative stress in blood leukocytes of rats with experimental diabetes
M. M. Guzyk1, K. O. Dyakun1, L. V. Yanitska2, Т. М. Kuchmerovska1
1Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: kuch@biochem.kiev.ua;
2O. O. Bogomolets’ National Medical University, Kyiv, Ukraine;
The study was undertaken to investigate the influence of specific inhibitors of poly(ADP-ribose)polymerase-1 (PARP-1), in particular nicotinamide and 1,5-isoqinolinediol on white blood cells of rats with diabetes. Using the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate ROS production in leukocytes was asseced. It was found that the development of streptozotocin-induced diabetes was accompanied by an intensification of oxidative stress and a significant decrease in viability of blood leukocytes as compared to control animals. Administration of PARP-1 inhibitors prevented the development of oxidative stress in leukocytes and increased their viability. It was shown a reduction of superoxide dismutase activity in serum in diabetes. Investigated PARP-1 inhibitors had no effect on the activity of superoxide dismutase and glucose levels in the blood. The findings suggest the intensification of oxidative stress in leukocytes of diabetic animals and the ability of nicotinamide and 1,5-isoqinolinediol to prevent its development depending on the features of their structure.
The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro
A. K. Gulevsky, N. N. Moisieieva, O. L. Gorina,
J. S. Akhatova, A. A. Lavrik, A. V. Trifonova
Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkiv;
e-mail: moiseeva-nataly@rambler.ru
The influence of a low-molecular fraction (below 5 kDa) from the cattle cord blood (CBF) on functional activity of phagocytes, human embryonic fibroblasts, mesenchymal stromal cells and BHK-21 clone 13/04 and PK-15 cells was studied. The low-molecular fraction added to culture medium increases the growth rate of cell cultures. The incubation of leukoconcentrate in the CBF-containing medium results in an increase in phagocytic indices of neutrophils in the presence of a phagocytosis inhibitor – sodium iodoacetate, leading to a significant increase in intracellular glucose content and alkaline phosphatase activity as compared to the control and the reference drug Actovegin®.