Ukr.Biochem.J. 2025; Volume 97, Issue 4, Jul-Aug, pp. 94-101

doi: https://doi.org/10.15407/ubj97.04.094

Influence of lipopolysaccharides of Escherichia coli on the protease activity of several Bacillus strains

10.09.2025   Uncategorized   No comments

L. D. Varbanets1, O. S. Brovarska1, O. V. Gudzenko1*,
K. G. Garkava2, A. R. Makarenko2

1Institute of Microbiology and Virology named after D. K. Zabolotny,
National Academy of Sciences of Ukraine, Kyiv, Ukraine;
2National Technical University of Ukraine
“Igor Sykorsky Kyiv Polytechnic Institute”, Kyiv, Ukraine;
*e-mail: alena.gudzenko81@gmail.com

Received: 07 July 2025; Revised: 23 August 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025

We have previously shown that lipopolysaccharides (LPS) of a number of strains of the phytopathogenic species Pantoea agglomerans are capable of increasing the activity of Bacillus peptidases with fibrinolytic, elastase and collagenase activities by 2-4 times. The aim of this work was to investigate the effect of isolated intracellular LPS1 and extracellular LPS2 of Escherichia coli on the activity of purified bacilli proteases with elastase and fibrinogenolytic activity. It was shown that both LPS2 and LPS1 of E. coli 23 can increase the elastase activity of Bacillus sp. IMV B-7883 by 600 and 416% respectively. Both LPS are able to increase fibrinogenolytic activity in all studied Bacillus strains, but its greatest stimulation (200%) was observed under the action of LPS2 of Bacillus sp. L9.

Keywords: , , , ,

References:

  1.  Raetz CR, Whitfield C. Lipopolysaccharide endotoxins. Annu Rev Biochem. 2002:71:635-700. PubMed, PubMed, CrossRef
  2.  Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science. 1998;282(5396):2085-2088. PubMed, cr id=”https://doi.org/10.1126/science.282.5396.2085″]
  3.  Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA. The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell. 1996;86(6):973-983. PubMed, CrossRef
  4.  Farhana A, Khan YS. Biochemistry, Lipopolysaccharide. In: StatPearls. Treasure Island (FL): StatPearls Publishing; April 17, 2023.
  5.  Kramer RA, Brandenburg K, Vandeputte-Rutten L, Werkhoven M, Gros P, Dekker N, Egmond MR. Lipopolysaccharide regions involved in the activation of Escherichia coli outer membrane protease OmpT. Eur J Biochem. 2002;269(6):1746-1752. PubMed, CrossRef
  6. Dzyublyuk NA, Varbanets LD, Bulyhina TV. Influence of Pantoea agglomerans lipopolisaccharides on the activity of Bacillus proteases. Microbiol Zh. 2018;80(1):27-35. (In Ukrainian). CrossRef
  7. Varbanets LD, Brovarskaya ОS, Garkava KG, Tymoshenko UV. Characteristics of Escherichia coli K Lipopolysacharide. Mikrobiol Zh. 2024;86(6):20-29. CrossRef
  8. Gudzenko OV, Varbanets LD, Butsenko LM, Pasichnyk LA, Chernyshenko VO, Stogniy EM. Strain Bacillus sp. IMV B-7883 – producer of extracellular proteinase with fibrinogenolytic activity. Utility patent No. 145577. Registered in the State Register of Patents of Ukraine for Utility Models. 28.12. 2020.
  9. Gudzenko OV, Varbanets LD. Screening of protease producers among representatives of the genus Bacillus isolated from the coastal zone of the Kinburn split. Microbiol Zh. 2024;86(2):3-9. CrossRef
  10. Ivanytsia VO, Shtenikov MD, Ostapchuk AM. Facultatively-anaerobic endosporeforming bacteria of deep water bottom sediments of Black Sea. Microbiol Biotechnol. 2017;(4):94-103.
  11. Westphal O, Jann K.Bacterial lipopolysaccharides extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem. 1965;5:83-91.
  12. Chaby R, Charon D, Caroff M, Sarfati SR, Trigalo F. Estimation of 3-deoxy-D-manno-2- octulosonic acid in lipopolysaccharides: an unsolved problem. In: Methods in Carbohydrate Chemistry. Ed. by BeMiller JN, Whistler RL, Shaw DH. NeW York: John Wiley J. & Sons. Inc, 1993;9: 33-46.
  13. Dische Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953;204(2):983-997. PubMed, CrossRef
  14. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28(3);350-356. PubMed, CrossRef
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-275. PubMed, CrossRef
  16. Spirin AS. Spectrophotometric determination of total nucleic acids. Biokhimiia. 1958;23(5):656-662. (In Russian). PubMed
  17. Albersheim P, Nevins DJ, English PD, Karr A. A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohydr Res. 1967;5(3):340-345. CrossRef
  18. Ouchterlony О. Diffusion-In-Gel Methods for Immunological Analysis II (Part 4 of 4). Chem Immunol Allergy. 1962:126-154.
    CrossRef
  19. Varbanets LD, Matseliukh EV. Peptidases of microorganisms and methods of their investigations. Kyiv, Naukova Dumka, 2014. 323 p.
  20. Masada M. Determination of the thrombolytic activity of Natto extract. Food style. 2004; 8 (1): 92-95.
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