Tag Archives: Bacillus

Influence of lipopolysaccharides of Escherichia coli on the protease activity of several Bacillus strains

10.09.2025   Uncategorized   No comments

L. D. Varbanets1, O. S. Brovarska1, O. V. Gudzenko1*,
K. G. Garkava2, A. R. Makarenko2

1Institute of Microbiology and Virology named after D. K. Zabolotny,
National Academy of Sciences of Ukraine, Kyiv, Ukraine;
2National Technical University of Ukraine
“Igor Sykorsky Kyiv Polytechnic Institute”, Kyiv, Ukraine;
*e-mail: alena.gudzenko81@gmail.com

Received: 07 July 2025; Revised: 23 August 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025

We have previously shown that lipopolysaccharides (LPS) of a number of strains of the phytopathogenic species Pantoea agglomerans are capable of increasing the activity of Bacillus peptidases with fibrinolytic, elastase and collagenase activities by 2-4 times. The aim of this work was to investigate the effect of isolated intracellular LPS1 and extracellular LPS2 of Escherichia coli on the activity of purified bacilli proteases with elastase and fibrinogenolytic activity. It was shown that both LPS2 and LPS1 of E. coli 23 can increase the elastase activity of Bacillus sp. IMV B-7883 by 600 and 416% respectively. Both LPS are able to increase fibrinogenolytic activity in all studied Bacillus strains, but its greatest stimulation (200%) was observed under the action of LPS2 of Bacillus sp. L9.

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Exometabolites of endospore-forming bacteria of Bacillus genus identified by genomic-metabolomic profiling

17.12.2020   Uncategorized   No comments

А. M. Ostapchuk, М. D. Shtenikov*, V. О. Ivanytsia

Odesa I. I. Mechnykov National University, Ukraine;
*e-mail: shtenikovnik@gmail.com

Received: 30 March 2020; Accepted: 13 November 2020

The set of unique bioactive metabolites produced by marine bacilli is already known but the metabolomic of these bacteria  is underinvestigated. The aim of this work was to carry out  the comparative analysis of metabolomic and genomic traits of Bacillus velezensis ONU 553, Bacillus pumilus ONU 554, Bacillus subtilis ONU 559 strains isolated from Black Sea bottom sediments. Organic extracts of each strain were analyzed using high-resolution liquid chromatography-mass spectrometry. General annotation of genomes was performed using PATRIC, search for secretory signals in the primary structure of selected proteins with using Signal IP analysis.  The search of biosynthetic gene clusters was performed using antiSMASH, PRISM 3 and BiG-SCAPEs, the reconstruction of metabolites- with PRISM 3 and TransATor analyzes. The study allowed to found and identified 90, 33 and 43 metabolites in the strains Bacillus velezensis ONU 553, Bacillus pumilus ONU 554 and  Bacillus subtilis ONU 559 respectively. The compounds found in metabolome were subdivided into two groups: those which are known members of the genus Bacillus and those new to both genus and prokaryotes in general. Among the secondary metabolites of studied strains the variants of the nonribosomal peptide class  surfactins (anhteisoC16-surfactin, surfactin B2-me ester), gageostatins, fengycins and amicoumacins, and the secreted protease inhibiting pentapeptide GPFPI were identified. The biosynthetic clusters of lipopeptides of the pumilacidin subgroup and amicoumacin antibiotic AI-77A were identified for the first time with the use of  bioinformatic tools. The data obtained replenish the  understanding of the marine bacilli biosynthetic potential.

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