L. Kot*, V. Konopelnyuk, K. Dvorshchenko, V. Vereschaka

ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: kot_lora@ukr.net

Received: 10 August 2020; Accepted: 17 May 2021

Metabolic disorders under conditions of obesity are known to be accompanied by systemic inflammation and immune system imbalance while nitric oxide (NO) system is one of the main key links in the immune responses regulation. The aim of the research was to evaluate NO-synthase (NOS) activity and NO content in the cells of thymus and spleen of rats with experimental obesity induced by 14 weeks of high-calorie diet. NO content in cell suspensions was determined using Griess reaction, NOS activity was estimated by NADPH⁺+Н⁺ specific cleavage. It was shown that under conditions of experimental obesity NO content in cells of thymus and spleen as well as NOS activity in the spleen were decreased with  simultaneous increase in both NO content and NOS activity in the serum. The obtained data testify the changes in lymphoid cells functional activity and in organism general adaptive capacity under conditions of obesity.