L. T. Mishchenko¹*, O. О. Molodchenkova²*, A. A. Dunich¹,
I. A. Mishchenko³, A. V. Dashchenko³, P. S. Tykhonov²,
I. I. Motsniy², Ya. S. Fanin²

¹Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”, Ukraine;
*e-mail: lmishchenko@ukr.net;
²Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation,
Laboratory of Plant Biochemistry, Odesa, Ukraine;
*e-mail: olgamolod@ukr.net;
³National University of Life and Environmental Sciences of Ukraine, Kyiv

Received: 11 May 2025; Revised: 20 August 2025;
Accepted: 30 January 2026; Available on-line: February 2026

Soybean mosaic virus (SMV) infection is recognized as the most serious, long-standing problem in soybean producing areas in the world. The Rsv1 locus is a part of resistance genes family involved in plant defense mechanisms against pathogens. Rsv1 and antioxidant enzymes peroxidase and superoxide dismutase are known for their role in providing resistance to the soybean mosaic virus. This work aimed at screening soybean plants both SMV infected and healthy on the presence of Rsv1 locus and peroxidase and superoxide dismutase isozyme patterns. The presence of 3gG2 gene at the Rsv1 locus was detected with PCR in uninfected plants of 17 studied soybean varieties. The presence of 3gG2 gene was also revealed in SMV-infected plants of four varieties indicating that the gene was not expressed in these plants. electrophoresis in PAAG demonstrated that the spectrum of peroxidase and superoxide dismutase isoforms in soybean leaf tissues depends on genotype specificity and the presence/absence of the 3gG2 gene at the Rsv1 locus. The outcome of work will be useful for identification genotypes resistant to SMV and their implementation in soybean breeding programs in Ukraine.