Tag Archives: protein phosphorylation

Bioinformatic analysis of potential post-translational modification sites of the human O(6)-methylguanine-DNA methyltransferase (MGMT) protein

A. P. Iatsyshyna1, Z. M. Nidoieva2, O. V. Pidpala1, L. L. Lukash1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: a.p.iatsyshyna@imbg.org.ua;
2M. P. Drahomanov National Pedagogical University, Kyiv, Ukraine

Using in silico analysis a number of potential sites for post-translational modifications has been revealed within the human O6-methylguanine-DNA methyltransferase (MGMT) protein. In particular these were the acetylation of Gly3 residue in the N-terminus of protein and internal residues Lys132 and Lys135; Arg166 residue methy­lation; Lys63 SUMOylation and ubiquitination of Lys31, Lys39, Lys49, Lys63, Lys67, Lys135, Lys156, Lys196, Lys209. Also it has been predicted 16 novel potential phosphorylation sites of serine residues (positions 13, 124, 144, 182, 183, 190, 215, 216 and 230), tyrosine residues (positions 100 and 189) and threonine residues (positions 23, 69, 94, 126 and 229), as well as five binding sites for kinases and other proteins (Ser13 with 14-3-3, Val21 and Ile172 with D-domain, Pro78 and Pro111 with SH3-domain, Pro111 with MAPK3). Some kinases predicted by the authors are known as partners of the MGMT protein, that confirms the probability of modification of the given sites. Potential sites require further experimental confirmation of modi­fications and investigation of their influence on stability and DNA-repair activity of this protein.

Laureates of the Palladin Prize of the National Academy of Sciences of Ukraine (2005, 2007 years)

R. P. Vynogradova ,  V. M. Danilova 

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua

The article presents biographical information and analysis of scientific activity of laureates of the Palladin Prize of NAS of Ukraine. In 2005 L. I. Ostapchenko and A.A. Sibirny were awarded for the series of works “Molecular mechanisms of metabolism regulation and their application  in biology and biotechnology” and in 2007 O. G. Minchenko received the prize for the series of works “Molecular mechanisms of regulation of gene expression” published between 2001 and 2005.