Tag Archives: reduce glutathione

Effects of L-glutamic acid and pyridoxine on glutathione depletion and lipid peroxidation generated by epinephrine-induced stress in rats

N. O. Salyha

Institute of Animal Biology, NAAS of Ukraine, Lviv;
е-mail: ynosyt@yahoo.com

The main goal of this research was to investigate and compare the protective effects of L-glutamic acid (L-Glu) alone and combined with Pyridoxine (L-Glu+Pyridoxine) for the purpose of suppression and mitigation of epinephrine-induced stress in rats. This study outlines possible links between changes of reduced glutathione (GSH) level, antioxidant enzymes activity and content of the lipid peroxidation products after administration of the above-mentioned substances and under the action of stress in various tissues of rats. The obtained results suggest that the GSH level was significantly inhibited by stress in all investigated tissues (except kidneys). We have shown that under the stress, activities of glutathione-associated enzymes were changed (mainly decreased) in all investigated tissues. In rats, additionally received L-Glu and L-Glu+Pyridoxine, much less changes or lack of changes in studied parameters were observed. The content of lipid peroxidation products (lipid peroxides (LOOH) and thiobarbituric acid reactive substances (TBARS)) in myocardium, liver and kidney tissues of experimental groups under the stress conditions were significantly higher compared to the control. While in experimental groups that received L-Glu and L-Glu+Pyridoxine LOOH content in kidney, spleen and liver and TBARS content in spleen, liver and myocardium were almost at the level of control values. These results indicate that L-Glu and L-Glu+Pyridoxine can mitigate and suppress epinephrine-induced stress in rats.