Tag Archives: RNA-interference

RNA interference of proteasome subunit of PSMβ7 gene restricts proteasome subunit PSMβ1 and PSMβ5 mRNA expression and peptidyl-glutamyl peptide-hydrolyzing proteasome activity in neonatal cardiomyocytes

V. O. Kyrychenko, D. O. Pashevin, L. V. Tumanovska, V. E. Dosenko, O. O. Moibenko

Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: victoria30@mail.ru

Using small interfering RNA (siRNA) transfection of neonatal cardiomyocytes to inhibit expression of nonproteolytic proteasome β7 subunit, we observed a significant decrease in β1 proteolytic subunit mRNA expression. Proteasome peptidyl-glutamyl peptide-hydrolyzing activity decreased to 28% (0.48 ± 0.2 nM AMC/min) compared to control (1.7 ± 0.5 nM AMC/min) (Р < 0.05). β5 Subunit mRNA expression decreased 21 times (Р < 0.05) with no changes in its chymotrypsin-like activity. Proteasome trypsin-like activity and activity of another proteolytic enzyme tripeptidylpeptidase II remained unchanged.

Specific silencing of leukemic oncogenes using RNA-interference approach

T. D. Lebedev1, P. V. Spirin1, N. N. Orlova1, A. S. Gornostaeva1, C. Stocking2, V. S. Prassolov1

1Engelghardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia;
2Heinrich-Pette Institute of Experimental Virology and Immunology, Hamburg, Germany;
e-mail: prassolov45@mail.ru

RNA-interference is an effective natural mechanism of post-transcriptional modulation of gene expression. RNA-interference mechanism exists as in high eukaryotes both animals and plants as well in lower eukaryotes and viruses. RNA-interference is now used as a powerful tool in the study of functional gene activity,  and many essential for fundamental biology results were obtained with this approach. Also it is widely believed that RNA-interference could be used in working out of new therapeutic medicine against malignant, infectious and hereditary diseases. One of the main problems of these developments is search of effective methods of siRNA transfer into the target cells. At the present time different sorts of transfections or viral transduction are used for these purposes. The results of comparison of inhibition of expression of oncogene AML-ETO by synthetic siRNA and by recombinant lentiviruses coding for corresponding shRNA are presented in the article.