Tag Archives: vitamins
Assessing the in vitro anti-glycation efficacy of vitamins A, C, D, E
N. P. Dalbanjan1, A. J. Kadapure1, P. Huded2,
V. B. Chachadi1, S. Nayaka3, Praveen Kumar S.K.1*
1Department of Biochemistry, Karnatak University, Dharwad, Karnataka, India;
2FS and AQCL Department CSIR-CFTRI Mysore 570020, Karnataka, India;
3Department of Botany, Karnatak University, Dharwad, Karnataka, India;
*e-mail: praveenkumarsk@gmail.com; praveenkumarsk@kud.ac.in
Received: 27 June 2022; Revised: 31 August 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022
Accreted sugars in the blood react with protein’s amino group via Schiff base to form Amadori compounds. Further, these compounds execute irreversible chemical modifications generating advanced glycation end products (AGEs). The current study investigated the effects of vitamins in a glycation-prone in vitro environment. Glycation model was reached by incubating BSA with 0.1 M glucose/fructose in 0.1 M phosphate-buffered saline. Intrinsic (tyrosine/tryptophan) and AGEs fluorescence was monitored with fluorescence spectrophotometer. Ellman’s test depicted that native BSA contains more free thiol groups than glycated BSA. It was shown that BSA is more susceptible to glycation in the presence of fructose than glucose, and vitamin D followed by vitamin E and A can significantly rescue the BSA from glycation progression.
The contribution of the Nobel prize laureates to the development of knowledge of vitamin biochemistry: Ch. Eijkman, F. G. Hopkins, A. Szent-Györgyi, W. Haworth, P. Karrer, R. Kuhn, H. Dam, E. A. Doisy, G. Minot, W. Murphy, G. Whipple, D. Hodgkin, R. Woodward
V. M. Danilova, R. P. Vynogradova, S. V. Komisarenko
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua
In the first half of the 20th century, the experimental research of chemists, biochemists and physiologists in collaboration with doctors led to the discovery of a new class of biologically active compounds – vitamins. Many of these scientists were awarded the Nobel Prizes. Thanks to their efforts, almost all currently known vitamins (B1, B2, B6, B9, B12, C, A, E, K) were identified, their structure and the mechanism of biological action were characterized. Many vitamins were found to serve as coenzymes in important biochemical conversions. This article talks about the history of the discovery of the most familiar vitamins and scientists involved in their research. The contribution made by these distinguished scientists to the development of modern biochemical science, in particular, vitaminology, cannot be overestimated.
Analysis of inventive activity of the department of vitamin and coenzyme biochemistry of Palladin Institute of Biochemistry NAS of Ukraine in the period of 1980-2015
V. M. Danilova, I. G. Chernysh, R. P. Vynogradova, G. G. Lugovska, S. P. Yurasova
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiyv;
e-mail: valdan@biochem.kiev.ua
The article provides analysis of inventive activity of the department of vitamin and coenzyme biochemistry of Palladin Institute of Biochemistry NAS of Ukraine in the years 1980-2015. It is pointed out that since 1985 fundamentally novel data on molecular mechanisms of coenzyme and non-coenzyme effect of vitamins and their nature metabolites were obtained in the Department headed by Prof., Corresponding Member of NAS of Ukraine G. V. Donchenko. Based on the obtained experimental data innovative technologies as well as novel products for medicine, agriculture, food and pharmaceutical industries were developed. Nearly 40 patents and authorship certificates of USSR and Ukraine for these practical developments were granted to the department employees.