Ukr.Biochem.J. 2013; Volume 85, Issue 3, May-Jun, pp. 69-73


Adjuvant properties of polymer based on acrylic acid

M. R. Kozak1, A. V. Oliynyk1, O. S. Zaichenko2, V. V. Vlizlo1

1Institute of Animal Biology, National Academy of Agrarian Sciences of Ukraine, Lviv;
2Lviv Polytechnic National University, Ukraine;

Adjuvant properties of the polymer containing­ acrylic acid, glycidyl methacrylate, triethylene glycol methacrylate and butyl acrylate have been established. Antibodies to ovalbumin and bovine serum albumin in the blood of mice were revealed using dot blot analysis and immunoenzyme analysis when applying the investigated polymer as a carrier of these protein antigens. Adjuvant properties of the polymer were compared to aluminum hydroxide, which is a component of many traditional vaccines. Experimental polymer was a stronger adjuvant because it led to an increase of specific antibodies against ovalbumin and bovine serum albumin.

Keywords: , , ,


  1. Glik B, Pasternak J.  Molecular Biotechnology. Principles and Applications. Ed. N. K. Yavorsky, transl. from english. M.: Mir, 2002. 589 p.
  2.  Bittle JL, Houghten RA, Alexander H, Shinnick TM, Sutcliffe JG, Lerner RA, Rowlands DJ, Brown F. Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature. 1982 Jul 1;298(5869):30-3. PubMed, CrossRef
  3. Spazierer D, Skvara H, Dawid M, Fallahi N, Gruber K, Rose K, Lloyd P, Heuerding S, Stingl G, Jung T. T helper 2 biased de novo immune response to Keyhole Limpet Hemocyanin in humans. Clin Exp Allergy. 2009 Jul;39(7):999-1008. PubMed, CrossRef
  4. Lindblad EB. Aluminium compounds for use in vaccines. Immunol Cell Biol. 2004 Oct;82(5):497-505. Review. PubMed, CrossRef
  5.  Baylor NW, Egan W, Richman P. Aluminum salts in vaccines–US perspective. Vaccine. 2002 May 31;20 Suppl 3:S18-23. Review. PubMed, CrossRef
  6.  Lindblad EB. Aluminium adjuvants–in retrospect and prospect. Vaccine. 2004 Sep 9;22(27-28):3658-68. Review. PubMedCrossRef
  7. Martyn CN, Osmond C, Edwardson JA, Barker DJ,  Harris EC,  Lacey RF. Geographical relation between Alzheimer’s disease and aluminum in drinking water. Lancet. 1989 Jan 14;1(8629):59-62. PubMed, CrossRef
  8. Foged C, Brodin B, Frokjaer S, Sundblad A. Particle size and surface charge affect particle uptake by human dendritic cells in an in vitro model. Int J Pharm. 2005 Jul 25;298(2):315-22. PubMed, CrossRef
  9. Kanchan V, Panda AK. Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response. Biomaterials. 2007 Dec;28(35):5344-57. PubMed, CrossRef
  10. Brunner R, Jensen-Jarolim E, Pali-Schöll I. The ABC of clinical and experimental adjuvants–a brief overview. Immunol Lett. 2010 Jan 18;128(1):29-35. Review. PubMed, PubMedCentral, CrossRef
  11. Moskvin M., Oliynyk A., Mitina N. et al. New porous peroxide containing gel colloids  and nanoparticle of polyelectrolyte nature.  VI Scientific and technical conference “Progress in the oil/gas refining and petrochemical industries”. Lviv. April 25-28, 2012. p. 236.
  12. Stieneker F, Kreuter J, Löwer J. High antibody titres in mice with polymethylmethacrylate nanoparticles as adjuvant for HIV vaccines. AIDS. 1991 Apr;5(4):431-5. PubMed, CrossRef
  13. Salman HH, Gamazo C, de Smidt PC, Russell-Jones G, Irache JM. Evaluation of bioadhesive capacity and immunoadjuvant properties of vitamin B(12)-Gantrez nanoparticles. Pharm Res. 2008 Dec;25(12):2859-68. PubMed, CrossRef
  14. Salman HH, Irache JM, Gamazo C. Immunoadjuvant capacity of flagellin and mannosamine-coated poly(anhydride) nanoparticles in oral vaccination. Vaccine. 2009 Jul 30;27(35):4784-90. PubMed, CrossRef
  15. Oliynyk AV, Kozak MR, Zaichenko OS, Vlizlo VV. Lipid hydroperoxides and tba-active products in mouse organs at the action of adjuvants – polymer mg-4 and aluminum hydroxide. Animal Biology. 2012;14(1-2):174-179.
  16. Laboratory methods of research in biology, animal husbandry and veterinary medicine. Ed. V.V. Vlizlo. Lviv: Spolom, 2012. 761 p.

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.