Ukr.Biochem.J. 2013; Volume 85, Issue 5, Sep-Oct, pp. 170-176


Identification of components of the milky juice of Lactarius pergamenus (Fr.)Fr fungi by gas-liquid chromatography/mass spectrometry

M. V. Tsivinska1,2, L. V. Panchak3, R. S. Stoika4,1, V. O. Antonyuk4,3

1Ivan Franko Lviv National University, Ukraine;
2Scientific-Research Center of Criminalistic Examination in Lviv Region, Ministry of Internal Affairs of Ukraine;
3Danylo Halytskyi Lviv National Medical University, Ukraine;
4Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;

The authors have proposed method of separation of methanol extract of Lactarius pergamenus basidiomes and investigation of fractions extracted with hexane which, according to our previous studies, possess the highest antiproliferative and antifungal activity. Main attention was given to fractionation and analysis by gas-liquid chromatography-mass spectrometry (GLC/MS) of the fraction 1.4 extracted with hexane. The key role in separation of this fraction was played by the use of dioxane which mixes well with both polar and nonpolar solvents (hexane, chloroform, methanol, water). Chemical composition of hexanoic fraction of methanol extract of dried basidomes Lactarius pergamenus fungi were completely characterized. It was found that this fraction  consisted of 38% of fatty acids and their derivatives, 29% – of the phthalates, 13% – of the sesquiterpene, 2% – of aldehydes and 18% – of other compounds (hydrocarbons, alcohols, hydrazine derivatives and unidentified substances). Such combination of constituents allows forming a stable emulsion of milky juice which protects the mushroom fruit body from the bacterial and fungal infections and from eating by the mammalians and insects.

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  1. Kramer R. Abraham W.-R.  Volatile sesquiterpenes from fungi: what are they good for? Phytochem Rev. 2012 March;11(1):15-37.  CrossRef
  2. Velíšek J., Cejpek K. Pigments of higher fungi: a review. Czech J Food Sci. 2011;29(2):87-102.
  3. Zhou ZY, Liu JK. Pigments of fungi (macromycetes). Nat Prod Rep. 2010 Nov; 27(11):1531-70. CrossRef
  4. Jonassohn M. Sesquiterpenoid unsaturated dialdehydes. Structural properties that affect reactivity and bioactivity.  Doctoral Thesis. Lund University (Sweden): Lund, 1996. 83 р.
  5. Hansson T., Pang Z., Sterner O. The Conversion of [12-2H3]-Labelled Velutinal in Injured Fruit Bodies of Lactarius vellereus. Further Insight into the Biosynthesis of the Russulaceae Sesquiterpenes.  Acta Chemia Scandinavica. 1993;47:403-405. CrossRef
  6. Panchak LV, Klyuchivska OYu, Tsivinska MV, Stoika RS, Lesyk RB, Antonyuk VO. The chemical composition and antiproliferative activity of fractions of the methanol extract from the basidiomes of Lactarius pergamenus (Fr.)Fr. Biotekhnolohiia. 2012;5(1):78-85.
  7. Panchak LV, Tsivinska MV, Antonyuk VO, Stoika RS. Chemical composition of the frozen out methanol extracts from true mushrooms basidioms. Biotekhnolohiia. 2011;4(5):90-96.
  8. Pat. 54969 UA, ICP A61K9/06, A61K35/84 / Zaichenko O.I., Panchak L. V., Antoniuk V. O. et al.; Patent owner O.I. Zaichenko, L.V. Panchak, V.O. Antoniuk, O.O. Nemchenko, S.B. Goshkina, M.V.Tsivinska, R.S. Stoika, O.P. Korniychuk, V.V. Danyleichenko. № u 2010 08043; appl. 29.06.2010 ; Publ. 25.11.2010. Bul. N 22.
  9. Panchak LV, Antonyuk VO. Purification of a lectin from fruit bodies of Lactarius pergamenus (Fr.) Fr. and studies of its properties. Biochemistry (Mosc). 2011 Apr;76(4):438-49. PubMed, CrossRef

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