Ukr.Biochem.J. 2013; Volume 85, Issue 4, Jul-Aug, pp. 90-93

doi: http://dx.doi.org/10.15407/ubj85.04.090

Influence of metanandamide on steroidogenesis in rat adrenocorticocytes in vitro

28.10.2015   Uncategorized   No comments

N. I. Levchuk

State Institution “V. P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine”, Kyiv;
e-mail: levnataly@meta.ua

It is known from literature about antioxidant, anti-inflammatory, membrane protective and adreniregulatory properties of N-acetylethanolamines, but data concerning their participation in regulation of steroidogenesis are insufficient. In order to study the influence of a synthetic analogue of endogenous canabinoid anandamide – metanandamide – on the intensity of steroidogenesis the influence of different concentrations of the drug on the contents of 11-hydroxicorticosteroides (11-НСS) in the culture medium after incubation of adrenal tissue in rats of both sexes was investigated. The quantitative determination of 11-HСS was conducted by fluorometric micromethod. It was shown that the incubation of tissue sections with metanandamide leads to a reduction of 11-HСS in males and an increase of their content in females. It was concluded that the inhibition of corticosteroid secretion and synthesis in males may be due to reduction of cAMP and inhibition of cAMP-dependent protein kinase A (PKA) under the effect of metanandamide. The opposite and dose-dependent effects of the preparation in females may be connected with the estrogen influen­ce on the mechanisms of drug effect realization.

Keywords: , , ,

References:

  1. Hula NM, Margitych VM. Fatty acids and their derivatives under pathological conditions. K.: Naukova dumka, 2009. 334p.
  2. Mikosha A, Kovzun O, Zhukov A, Gulaya N. Effect of dopamine and long-chain N-acylethanolamines on streidogenesis in rat adrenal gland in vitro. Med Sci Res. 1998;26:85-88.
  3. Hula NM, Mikosha OS, Zhukov OD, Chelnakova IS. Effect of N-acylethanolamines on adrenal cortex function. Ukrainian. Ukr Biokhim Zhurn. 2000 May-Jun;72(3):82-6. PubMed
  4. Storozhuk LM, Zhukov O., Artamonov M.N. Pathways of N-acylethanolamine action on the rat adrenal cortex function. Endokrynologia. 2005;10(1):63-68.
  5. Weidenfeld J, Feldman S, Mechoulam R. Effect of the brain constituent anandamide, a cannabinoid receptor agonist, on the hypothalamo-pituitary-adrenal axis in the rat. Neuroendocrinology. 1994 Feb;59(2):110-2. PubMed, CrossRef
  6. Wenger T, Jamali KA, Juanéda C, Léonardelli J, Tramu G. Arachidonyl ethanolamide (anandamide) activates the parvocellular part of hypothalamic paraventricular nucleus. Biochem Biophys Res Commun. 1997 Aug 28;237(3):724-8. PubMed, CrossRef
  7. Manzanares J, Corchero J, Fuentes JA. Opioid and cannabinoid receptor-mediated regulation of the increase in adrenocorticotropin hormone and corticosterone plasma concentrations induced by central administration of delta(9)-tetrahydrocannabinol in rats. Brain Res. 1999 Aug 21;839(1):173-9. PubMed, CrossRef
  8. Balashov IuG. A fluorimetric micromethod for determining corticosteroids: a comparison with other methods. Fiziol Zh SSSR Im I M Sechenova. 1990 Feb;76(2):280-3. Russian. PubMed
  9. Zhukov OD. Effect of T-acylethanolamine on steroidogenesis in the adrenal glands. Avtoref. dis. … kand. biol. nauk. K.,2001. 16p.
  10. Kovzun OI, Levchuk NI, Hula NM, Mikosha OS. Participation of cyclic nucleotides, protein kinase A and C in realization of N-acylethanolamine action in human adrenocortical cells. Ukr Biokhim Zhurn. 2007 Sep-Oct;79(5):133-9. Ukrainian. PubMed
  11. Ziegler CG, Mohn C, Lamounier-Zepter V,  Rettori V, Bornstein SR,  Krug AW,  Ehrhart-Bornstein M.  Expression and function of endocannabinoid receptors in the human adrenal cortex. Horm Metab Res. 2010 Feb;42(2):88-92. PubMed, CrossRef
  12. Mikosha AS, Kovzun EI, Grinchenko EN. Estradiol-17beta activates corticosteroid synthesis and inhibits proliferation in cultured porcine adrenocortical cells. Ukr Biokhim Zhurn. 2003 Jan-Feb;75(1):29-32. Russian. PubMed
  13. Kovzun OI, Grinchenko EM, Mikosha OS. Direct and indirect effects of estradiol on glucocorticoid function of the adrenal cortex. Clin Exp Pathol. 2004;3(2, Part 1):137-139.
  14. Scorticati C, Fernández-Solari J, De Laurentiis A, Mohn C, Prestifilippo JP, Lasaga M, Seilicovich A, Billi S, Franchi A, McCann SM, Rettori V. The inhibitory effect of anandamide on luteinizing hormone-releasing hormone secretion is reversed by estrogen. Proc Natl Acad Sci USA. 2004 Aug 10;101(32):11891-6. PubMed, PubMedCentral, CrossRef
  15.  González S, Bisogno T, Wenger T, Manzanares J, Milone A, Berrendero F, Di Marzo V, Ramos  JA,  Fernández-Ruiz JJ. Sex Steroid Influence on Cannabinoid CB1 Receptor mRNA and Endocannabinoid Levels in the Anterior Pituitary Gland. Biochem Biophys Res Commun. 2000 Apr;270(1):260-266. PubMed, CrossRef
  16. El-Talatini MR, Taylor AH, Konje JC. The relationship between plasma levels of the endocannabinoid, anandamide, sex steroids, and gonadotrophins during the menstrual cycle. Fertil Steril. 2010 Apr;93(6):1989-96. PubMed, CrossRef
  17. Maccarrone M, Bari M, Battista N, Finazzi-Agrò A. Estrogen stimulates arachidonoylethanolamide release from human endothelial cells and platelet activation. Blood. 2002 Dec 1;100(12):4040-8. PubMed, CrossRef
  18. Maccarrone M, Bisogno T, Valensise H, Lazzarin N, Fezza F, Manna C, Di Marzo V, Finazzi-Agrò A. Low fatty acid amide hydrolase and high anandamide levels are associated with failure to achieve an ongoing pregnancy after IVF and embryo transfer. Mol Hum Reprod. 2002 Feb;8(2):188-95. PubMed, CrossRef
  19.  Maccarrone M, Falciglia K, Di Rienzo M, Finazzi-Agrò A. Endocannabinoids, hormone-cytokine networks and human fertility. Prostaglandins Leukot Essent Fatty Acids. 2002 Feb-Mar;66(2-3):309-17. Review. PubMed, CrossRef
  20. Guo Y, Wang H, Okamoto Y, Ueda N, Kingsley PJ, Marnett LJ, Schmid HH, Das SK, Dey SK. N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation. J Biol Chem. 2005 Jun 24;280(25):23429-32. Epub 2005 May 12. PubMed, CrossRef
  21. Lépicier P, Bouchard JF, Lagneux C, Lamontagne D. Endocannabinoids protect the rat isolated heart against ischaemia. Br J Pharmacol. 2003 Jun;139(4):805-15. PubMed, PubMedCentral, CrossRef
  22. De Petrocellis L, Orlando P, Di Marzo V. Anandamide, an endogenous cannabinomimetic substance, modulates rat brain protein kinase C in vitro. Biochem Mol Biol Int. 1995 Aug;36(6):1127-33. PubMed
  23. Grinchenko EN, Kovzun EI, Mikosha AS. Effect of 17beta-estradiol on content of cyclic nucleotides and protein kinases A and C activity in human adrenal cortex. Ukr Biokhim Zhurn. 2006 Sep-Oct;78(5):88-92. Russian. PubMed
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