Ukr.Biochem.J. 2013; Volume 85, Issue 5, Sep-Oct, pp. 149-153


Kinetic pattern of folic acid release from polyurethane matrices of varied structures

M. V. Grigorieva

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

The development of new bioactive polymeric materials is very topical. The goal of the present work was to use an optimized reversed-phase HPLC method to determine the release of folic acid (FA) from polyurethane carriers of different structures in vitro. Two types of polymeric samples were studied as FA carriers: linear (covalent bonds between FA and the carrier; 13 mass % and 26 mass % FA – samples 1 and 2) and cross-linked (hydrogen bonds between FA and the carrier; 1 mass % FA and 6 mass % FA – samples 3 and 4). The rate and pattern of the FA release was shown to depend on the way of FA immobilization on the polymeric carrier and the polymer structure. The cumulative amount of FA released from the linear samples (1 and 2) into model medium on the 28th day was 1.20 mg/ml and 2.17 mg/ml, respectively. The cumulative amount of FA released from the cross-linked samples (3 and 4) on the 28th day was 0.16 mg/ml and 0.38 mg/ml, respectively.

Keywords: , , , ,


  1. Jeong B, Bae YH, Lee DS, Kim SW. Biodegradable block copolymers as injectable drug-delivery systems. Nature. 1997 Aug 28;388(6645):860-2. PubMed, CrossRef
  2. Pinchuk L, Wilson GJ, Barry JJ, Schoephoerster RT, Parel JM, Kennedy JP. Medical applications of poly(styrene-block-isobutylene-block-styrene) (“SIBS”). Biomaterials. 2008 Feb;29(4):448-60. Review. PubMed, CrossRef
  3. Grigor’eva MV. Polymer systems with controlled release of bioactive compounds. Biotekhnolohiia. 2011;4(2):9-23.
  4. Enyedy EA, Farkas E, Dömötör O, Santos MA. Interaction of folic acid and some matrix metalloproteinase (MMP) inhibitor folate-γ-hydroxamate derivatives with Zn(II) and human serum albumin. J Inorg Biochem. 2011 Mar;105(3):444-53. PubMed, CrossRef
  5. Shishehbore MR, Sheibani A, Haghdost A. Kinetic spectrophotometric method as a new strategy for the determination of vitamin B9 in pharmaceutical and biological samples. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):304-7. Epub 2011 Jun 22. PubMed, CrossRef
  6. Nelson BC, Sharpless KE, Sander LC. Quantitative determination of folic acid in multivitamin/multielement tablets using liquid chromatography/tandem mass spectrometry. J Chromatogr A. 2006 Dec 1;1135(2):203-11. PubMed, CrossRef
  7. Kall MA. Determination of total vitamin B6 in foods by isocratic HPLC: a comparison with microbiological analysis. Food Chem. 2003 Aug; 82(2): 315-327. CrossRef
  8. Leung J, Dwyer J, Hibberd P, Jacques P, Rand W, Rocco MV; HEMO Study Group. Association between adherence to folic acid supplements and serum folate, and plasma homocysteine among hemodialysis patients. J Ren Nutr. 2011 May;21(3):246-56. PubMed, PubMedCentral, CrossRef
  9. Koufopantelis P, Georgakakou S, Kazanis M, Giaginis C, Margeli A, Papargiri S, Panderi I. Direct injection liquid chromatography/positive ion electrospray ionization mass spectrometric quantification of methotrexate, folinic acid, folic acid and ondansetron in human serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 15;877(30):3850-6. PubMed, CrossRef
  10. Deconinck E, Crevits S, Baten P, Courselle P, De Beer J. A validated ultra high pressure liquid chromatographic method for qualification and quantification of folic acid in pharmaceutical preparations. J Pharm Biomed Anal. 2011 Apr 5;54(5):995-1000. PubMed, CrossRef
  11. Jin P, Xia L, Li Z, Che N, Zou D, Hu X. Rapid determination of thiamine, riboflavin, niacinamide, pantothenic acid, pyridoxine, folic acid and ascorbic acid in Vitamins with Minerals Tablets by high-performance liquid chromatography with diode array detector. J Pharm Biomed Anal. 2012 Nov;70:151-7. PubMed, CrossRef
  12. Rivas A, Rodrigo D, Company B, Sampedro F,  Rodrigo M. Effects of pulsed electric fields on water-soluble vitamins and ACE inhibitory peptides added to a mixed orange juice and milk beverage. Food Chem. 2007;104(4):1550-1559.  CrossRef
  13. Breithaupt DE.  Determination of folic acid by ion-pair RP-HPLC in vitamin-fortified fruit juices after solid-phase extraction. Food Chem. 2001 Sep; 74(4):521-525. CrossRef
  14. Akhtar MJ, Khan MA, Ahmad I. High performance liquid chromatographic determination of folic acid and its photodegradation products in the presence of riboflavin. J Pharm Biomed Anal. 1997 Sep;16(1):95-9. PubMed, CrossRef
  15. Epshtein NA. Validation of HPLC techniques for pharmaceutical analysis (review). Pharmac Chem J. 2004;38(4):40-56. CrossRef
  16. Andryushina OS,  Zakashun TE,  Demchenko IB, Rozhnova RA.  Studying the dynamic release of folic acid from folate-containing polyurethane ureas. Dopovidi Nat Akad Nauk Ukrainy. 2011;(8):113-116.
  17. Grigorieva M, Mazur L, Galatenko N, Mnyshenko T, Zakashun T. A study on the properties of a polymeric composite based on network polyurethane having a long-lasting effect of narcotic drug antagonist. Polymer Zhurn. 2004;26(4):254-259.
  18. Westedt U, Wittmar M, Hellwig M, Hanefeld P, Greiner A, Schaper AK, Kissel T. Paclitaxel releasing films consisting of poly(vinyl alcohol)-graft-poly(lactide-co-glycolide) and their potential as biodegradable stent coatings. J Control Release. 2006 Mar 10;111(1-2):235-46. PubMed, CrossRef

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