Ukr.Biochem.J. 2013; Volume 85, Issue 6, Nov-Dec, pp. 129-133


Seeing is believing! Live confocal imaging of microvascular networks in situ: morphology, Ca(2+) signalling and tone

T. Burdyga, L. Borysova

Department of Cellular and Molecular Physiology,
Institute of Translational Medicine,
University of Liverpool, Crown Street, Liverpool, L69 3BX, UK

2- and 3-dimensional confocal imaging of Fluo-4 loaded ureteric microvesells in situ allowed us to demonstrate distinct morphology, Ca2+ signalling and contractility in myocytes of arcade arterioles and pericytes of arcade venules. In myocytes and pericytes, Ca2+ signals arise exclusively from Ca2+ release from the sarcoplasmic reticulum through inositol 1,4,5-trisphosphate receptors. Са2+ transients in pericytes are less oscillatory, slower and longer-lasting than those in myocytes. The data obtained suggest differences in the mechanisms controlling local blood flow in precapillary arterioles and postcapillary venules.

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