Ukr.Biochem.J. 2012; Volume 84, Issue 2, Mar-Apr, pp. 105-110

Analysis of titration curves of serum antibodies obtained by ELISA

S. A. Bobrovnik

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

Different forms of titration curves obtained by ELISA for serum antibodies, monoclonal antibodies­ and polyreactive immunoglobulins were considered. A new interpretation of dose-dependent­ titration curves was suggested. It was shown that our interpretation of dose-dependent titration curves for antibodies which are presented in some biological liquids allow obtaining additional information about properties of the samples. This information was not obtained earlier because of the wrong understanding of the considered problem.

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