А. K. Gulevsky, A. Yu. Nikolchenko

Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkov;
e-mail: lord_n2001@mail.ru

The influence of the penetrating cryoprotector and Mg²⁺ ions on the protein-synthesizing activity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors – ethylene glycol and DMSO – resulted in the concentration-dependant reversible inhibition of the protein biosynthesis and aminoacylation reaction in the cell-free system. These cryoprotectors at low concentrations intensified the stimulating effect of Mg²⁺ on the cumulative protein synthesis in the cell-free system.

Keywords: aminoacylation, ions Mg(2+), penetrating cryoprotectant, protein synthesis in the cell-free system