Ukr.Biochem.J. 2012; Volume 84, Issue 6, Nov-Dec, pp. 115-123

Characteristics of Ca(2+), Mg(2+)-ATPases of peripheral blood lymphocytes of patients with rheumatic pathology

R. V. Fafula, U. P. Efremova, Z. D. Vorobets

Danylo Halytski Lviv National Medical University, Ukraine;

The analysis of the kinetic properties of Ca2+, Mg2+-ATPase of saponin-perforated peripheral blood lymphocytes of donors and patients with rheumatoid arthritis and ankylosing spondylitis was carried out. When analyzing the alterations in hydrolase activity of Ca2+, Mg2+-ATPase it was shown that affinity of Ca2+, Mg2+-ATPase of plasma membrane and membranes of endoplasmic reticulum for ATP do not significantly differ. It was found that the inhibition of examined enzyme systems occurs by mixed type both due to the reduction of maximum reaction rate and to the decrease of Ca2+, Mg2+-ATPase affinity for ATP in conditions of rheumatic pathology in the immunocompetent cells. It was identified that Ca2+, Mg2+-ATPase had significantly lower affinity for Ca2+ in lymphocytes of persons with rheumatic disorders than in donors.

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