Ukr.Biochem.J. 2011; Volume 83, Issue 3, May-Jun, pp. 99-105
Comparative investigation by spectrofluorimetry and flow cytometry of plasma and inner mitochondrial membranes polarisation in smooth muscle cell using potential-sensitive probe DIOC(6)(3)
G. V. Danylovych, Yu. V. Danylovych, V. F. Gorchev
Palladine Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: danylovych@biochem.kiev.ua
Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3′-dihexyloxacarbocyanine [DiOC6(3)] has been demonstrated. The obtained results confirm the use of DiOC6(3) for studying the influence of effectors on transmembrane potentials of intact cell compartments.
Keywords: flow cytometry, myometrium cell, potential-sensitive probe, transmembrane potential
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