Ukr.Biochem.J. 2016; Volume 88, Issue 6, Nov-Dec, pp. 70-75


Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride

O. Ye. Akimov, V. O. Kostenko

Ukrainian Medical Stomatological Academy, Poltava, Ukraine;

In the article the function of nitric oxide (·NO) cycle in rat’s gastric mucosa was assessed under excessive combined chronic fluoride and nitrate intake during 30 days. It was estimated that general nitric oxide synthase activity (NOS) was increased during excessive sodium fluoride intake meanwhile influence on gene­ral nitrate reduction was not statistically significant, but general nitrite reduction was increased. General arginase activity decreased. Excessive sodium nitrate intake decreased NOS activity, but increased nitrate, nitrite reduction and general arginase activity. Combined sodium nitrate and fluoride intake increased NOS by 18.9%, nitrate reduction by 71.7%, nitrite by 161.5%, arginase activity increase by 61.4%. The highest amounts of peroxynitrite were obtained from excessive sodium fluoride intake group, excessive sodium nitrate intake showed the lowest levels and combined excessive sodium nitrate and fluoride intake levels had intermediate results. Summarizing the data obtained, the authors have made a conclusion that combined excessive sodium nitrate and fluoride intake creates optimal conditions for functioning of nitrate-nitrite reductases.

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  1. Morita M, Naito Y, Yoshikawa T, Niki E. Plasma lipid oxidation induced by peroxynitrite, hypochlorite, lipoxygenase and peroxyl radicals and its inhibition by antioxidants as assessed by diphenyl-1-pyrenylphosphine. Redox Biol. 2016 Aug;8:127-35. PubMed, PubMedCentral, CrossRef
  2. Lundberg JO, Weitzberg E, Gladwin MT. The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov. 2008 Feb;7(2):156-67. PubMed, CrossRef
  3. Jin L, Qin L, Xia D, Liu X, Fan Z, Zhang C, Gu L, He J, Ambudkar IS, Deng D, Wang S. Active secretion and protective effect of salivary nitrate against stress in human volunteers and rats. Free Radic Biol Med. 2013 Apr;57:61-7. PubMed, PubMedCentral, CrossRef
  4. Durante W, Johnson FK, Johnson RA. Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 2007 Sep;34(9):906-11. Review. PubMed, PubMedCentral, CrossRef
  5. Szabó C, Ischiropoulos H, Radi R. Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nat Rev Drug Discov. 2007 Aug;6(8):662-80. Review. PubMed, CrossRef
  6. Kaydashev IP, Nozhinova OA, Bobrova NA, Riabenko VV, Bondarenko VV, Kostenko VA, Gargovich AL. Apoptosis in the cells of parenchymatous organs in subacute sodium nitrate poisoning. Tsitol Genet. 2000 May-Jun;34(3):62-8. (In Russian). PubMed
  7. Jin L, Qin L, Xia D, Liu X, Fan Z, Zhang C, Gu L, He J, Ambudkar IS, Deng D, Wang S. Active secretion and protective effect of salivary nitrate against stress in human volunteers and rats. Free Radic Biol Med. 2013 Apr;57:61-7.  PubMed, PubMedCentral, CrossRef
  8. Amira S, Mulè F.  Effects of sodium fluoride on the mechanical activity in mouse gastric preparations. Can J Physiol Pharmacol. 2005 Apr;83(4):367-73. PubMed, CrossRef
  9. Stasiuk OA, Kostenko VO. Effect of peroxynitrite scavangers on oxidative processes in salivary gland tissues of white rats under sodium nitrate and fluoride combined excessive intake. Probl Ecol Med. 2012; 16(5-6):30-33. (In Ukrainian).
  10. Kmet TI, Vlasik LI. Nitrate-Cadmium intoxication in animals and protective effect tincture of Echinacea purpurea. Probl Nutrition. 2004; 3: 41-45. (In Ukrainian).
  11. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982 Oct;126(1):131-8. PubMed, CrossRef
  12. Koreshkov AP. Fundamentals of Analytical Chemistry, Volume 1. M.: Publishing house “Chemistry”. 1970. 472 p.
  13. Khramov VA, Listopad GG. Modification in the determination of ornithine by Chinard’s method and its use for quantitative determination of serum arginase. Lab Delo. 1973;10:591-2. (In Russian). PubMed
  14. Shraibman GN, Dyagileva EP, Skibina AV. Spectrophotometric methods of determination of the peroxynitrite and the nitrite. Bull Kemerovo State University. 2011;45:200-206. (In Russian).
  15. Sireli M, Bulbul A. The effect of acute fluoride poisoning on nitric oxide and methemoglobin formation in guinea pig. Turk J Vet Anim Sci. 2004; 28: 591-595.
  16. Wang Y, Loake GJ, Chu C. Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Front Plant Sci. 2013 Aug 16;4:314. PubMed, PubMedCentral, CrossRef

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