Ukr.Biochem.J. 2017; Volume 89, Issue 1, Jan-Feb, pp. 71-75

doi: https://doi.org/10.15407/ubj89.01.071

Age-related changes phospholipids of sterlet in liver and dorsal muscles

R. R. Suleimanova1, E. А. Hudz2, D. О. Melnychuk, L. H. Kalachniuk1

1National University of Life and Environmental
Sciences of Ukraine, Kyiv;
e-mail: kalachnyuk_liliya@nubip.edu.ua
2Palladin Institute of Biochemistry, National
Academy of Sciences of Ukraine, Kyiv

Study of phospholipids changes peculiarities in the liver and dorsal muscles of sterlet (Acipenser ruthenus Linnaeus) may be important to determine the etiology and pathogenesis of fatty liver. We  established that the content of total phospholipids in tissues of the liver and dorsal muscles of three-year-old sterlet was less than for two-year-old fish by 15% and 20% (P ≤ 0.01), respectively. The amount of phosphatidylcholine (P ≤ 0.05), phosphatidylethanolamine, phosphatidylserine (P ≤ 0.01), phosphatidylinositol (P ≤ 0.01) and cardiolipin in the liver of 3-year-old sterlet was lower than for the 2-year-old fish, while quantitative indices for lysophosphatidylcholine and sphingomyelin were slightly increased. Similarly, in the cells of the dorsal muscles, the amount of phospholipid components (except lysophosphatidylcholine) was decreased with age. A decrease in the amount of phosphatidylethanolamine and phosphatidylserine in the dorsal muscles of 3-year-old sterlet was significant. The major phospholipids respective distribution was stable, except for phosphatidylethanolamine and particularly sphingomyelin.

Keywords: , , ,


References:

  1.  Acipenser ruthenus (Sterlet). Regime of access: http://dx.doi.org/10.2305/IUCN.UK.2010-1.RLTS.T227A13039007.en.
  2. Vaskovskiy VE. Lipids. Soros Educat J. 1997;(3): 32-37.
  3. Hrytsyniak II, Smolianinov KB,Yanovych VH. Lipid metabolism in fish. Lviv, Triad plus, 2010. 335 p. (In Ukrainian).
  4. Moffat RG, Stamford B. Lipid metabolism and haelth, Taylor and Francis, 2006, 377 p.
  5. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PubMed
  6. Svetashev VI, Vaskovsky VE. A simplified technique for thin-layer microchromatography of lipids. J Chromatogr. 1972 May 3;67(2):376-8. PubMed, CrossRef
  7. Vaskovsky VE, Kostetsky EY, Vasendin IM. A universal reagent for phospholipid analysis. J Chromatogr. 1975 Nov 12;114(1):129-41. PubMed, CrossRef
  8. Keits M. Techniques of lipidology. Isolation and identification of lipid analysеs. Moscow: World, 1975. 322 p. (In Russian).
  9. Reznikov OH. General ethical principles of animal experimentation. First National Congress on Bioethics. Endocrinol. 2003; 8 (1): 142–145 (In Ukrainian).
  10. Orel NM. Lipid biochemistry. Minsk, 2007. 37 p. (In Russian).
  11. Gula NM, Margitich VM. Fatty acids and their derivatives in pathologic states. Kyiv: Naukova dumka, 2009. (In Ukrainian).
  12. Сardiolipin (phosphatidylglycerol). Biology and Medicine. Regime of access: http://medbiol.ru/medbiol/biochem/001cd687.htm.
  13. Quinn PJ. Sphingolipid symmetry governs membrane lipid raft structure. Biochim Biophys Acta. 2014 Jul;1838(7):1922-30. PubMed, CrossRef
  14. Brauweiler AM, Goleva E, Leung DY. Th2 cytokines increase Staphylococcus aureus alpha toxin-induced keratinocyte death through the signal transducer and activator of transcription 6 (STAT6). J Invest Dermatol. 2014 Aug;134(8):2114-21.  PubMed, PubMedCentral, CrossRef

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