Tag Archives: phospholipids

Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment

T. M. Horid’ko1, H. V. Kosiakova1, A. G. Berdyshev1, O. F. Meged1,
O. V. Onopchenko1, V. M. Klimashevsky1, О. S. Tkachenko1, V. R. Bazylianska1,
V. O. Kholin2, K. O. Peschana2, S. A. Mykhalskiy2, N. M. Hula1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: TanGoRi@ukr.net

The impairment of cognitive functions is the most studied medical and social problem nowadays. The aim of this study was to evaluate the protective effects of N-stearoylethanolamine (NSE) on memory state, blood and brain biochemical parameters in rats under scopolamine-induced cognitive impairment. The results of this study shown that NSE administration to rats per os (5 mg/kg, 5 days, during last 3 days NSE was administrated 20 min prior to scopolamine injection (1 mg/kg, once daily for 3 days, intraperitoneally)) prevented the development of memory impairment. In particular, NSE action was associated with the prevention of increase in acetylcholinesterase activity, changes in phospholipid, free and esterified cholesterol level in hippocampus and frontal cortex, and disruption in pro-/antioxidant balance in blood and studied brain sections. Considering the above mentioned biological effects, NSE is a promising drug candidate for integrative therapy of cognitive impairment of different profiles.

The effect of N-stearoylethanolamine on adipocytes free cholesterol content and phospholipid composition in rats with obesity-induced insulin resistance

O. S. Dziuba, Ie. A. Hudz, H. V. Kosiakova, T. M. Horid’ko, V. M. Klimashevsky, N. M. Hula

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: oksana.dziuba86@gmail.com

Obesity induces molecular changes that promote associated disorders, such as insulin resistance (IR) and type 2 diabetes. Low insulin sensitivity occurs primarily due to defects in the pathway of insulin action in target tissues, and there is a hypothesis that IR may originate in adipose tissue and is followed by dyslipidemia. In this study using methods of thin-layer and gas-liquid chromatography we investigated free cholesterol content and phospholipid composition of adipocytes of obesity-induced IR rats and its changes induced by the N-stearoylethanolamine (NSE) administration. The results we obtained demonstrated that free cholesterol content significantly increased in adipocytes of IR rats compared to control. The analysis of phospholipid composition indicated a reduction of phosphatidylcholine and the total content of phosphatidylinositol with phosphatidylserine, whereas the content of lysophosphatidylcholine, sphingomyelin and phosphatidylethanolamine increased in IR group compared to control. NSE administration caused a statistically significant decrease in total cholesterol level and had a considerable effect on normalization of individual phospholipids content. As far as NSE administration caused a statistically significant decrease in free cholesterol level and had a considerable effect on normalization of individual phospholipids content of adipocytes, we can consider NSE as a prospective compound worthy more complex investigation of its action under the pathological conditions.

Effects of thiosulfonates on the lipid composition of rat tissues

A. Z. Pylypets1,2, R. Ya. Iskra2, V. V. Havryliak1,2, A. V. Nakonechna1, V. P. Novikov1, V. I. Lubenets1

1Lviv Polytechnic National University, Ukraine;
2Institute of Animal Biology, NAAS of Ukraine, Lviv;
e-mail: vlubenets@gmail.com

Thiosulfonates are synthetic analogs of organic sulfur-containing compounds isolated from plants. Recent studies have shown that these substances lowering cholesterol content in the body, are effective against hyperlipidemia. Therefore, the aim of our investigation was to study the effect of synthesized thiosulfonates on the content of lipids and their spectrum in rats blood, liver and kidney. The amount of total lipids and their fractional profile were determined by thin-layer chromatography. The administration of methyl-, ethyl-, and allylthiosulfonates at a dose of 300 mg/kg of body weight did not cause significant changes in the content of total lipids and phospholipids, but led to the redistribution of their classes in the examined tissues. The content of triacylglycerols in the blood plasma under the action of ethyl- and allylthiosulfonates was decreased by 29.14 and 23.19% (P < 0.05-0.01), respectively, whereas the injection with methyl- and ethylthiosulfonates was accompanied by a significant decrease in mono-, di-, triglycerides and free fatty acids in the liver compared to control. The most significant changes in the lipid profile of kidney tissue were detected under the action of methylthiosulfonate.

Age-related changes phospholipids of sterlet in liver and dorsal muscles

R. R. Suleimanova1, E. А. Hudz2, D. О. Melnychuk, L. H. Kalachniuk1

1National University of Life and Environmental
Sciences of Ukraine, Kyiv;
e-mail: kalachnyuk_liliya@nubip.edu.ua
2Palladin Institute of Biochemistry, National
Academy of Sciences of Ukraine, Kyiv

Study of phospholipids changes peculiarities in the liver and dorsal muscles of sterlet (Acipenser ruthenus Linnaeus) may be important to determine the etiology and pathogenesis of fatty liver. We  established that the content of total phospholipids in tissues of the liver and dorsal muscles of three-year-old sterlet was less than for two-year-old fish by 15% and 20% (P ≤ 0.01), respectively. The amount of phosphatidylcholine (P ≤ 0.05), phosphatidylethanolamine, phosphatidylserine (P ≤ 0.01), phosphatidylinositol (P ≤ 0.01) and cardiolipin in the liver of 3-year-old sterlet was lower than for the 2-year-old fish, while quantitative indices for lysophosphatidylcholine and sphingomyelin were slightly increased. Similarly, in the cells of the dorsal muscles, the amount of phospholipid components (except lysophosphatidylcholine) was decreased with age. A decrease in the amount of phosphatidylethanolamine and phosphatidylserine in the dorsal muscles of 3-year-old sterlet was significant. The major phospholipids respective distribution was stable, except for phosphatidylethanolamine and particularly sphingomyelin.

Practical development of laboratory of biologics technology of the Palladin Institute of Biochemistry of NAS of Ukraine for the period 1991-2010

G. G. Lugovska, R. P. Vynogradova, N. E. Lugovska,
I. G. Chernysh, S. P. Yurasova, V. M. Danilova

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: tto@biochem.kiev.ua

The paper presents the results of inventive activity of the Laboratory of Biologics Technology of the Palladin Institute of Biochemistry, NAS of Ukraine, under the leadership of Z.M.Datsenko, Cand.Sc. (Biol.), during 1991-2010. The laboratory researchers have developed new technologies for production of medicines of lipid-protein nature, which are based on endogenous complexes of biologically active substances included in the composition of biomembranes of different origin. The researchers have created new technologies for drug PANTOCRINE (for injections and oral administration) from antlers of deers and horns of farm animals that have significantly higher biological activity compared with the commercial drug.  They have also created new biologically active drugs from antlers with a specific action: HIPPOCAMP, reducing blood pressure in various forms of hypertension, and PANTERON – biological regulator of synthesis of steroid hormones. Further the researchers have developed technologies for obtaining biologically active complexes of various specific action from marine organisms (calamari, clams mussels, shellfish): CALMOFIL and MOLUFIL, therapeutic agents for replacement therapy of surfactant system of the lungs; FILOMEK, the agent for prophylaxis and treatment of a human reproductive system disorders; MOLUSTERON, the glicolipopeptide complex, three individual physiologically active compositions being differentially obtained from the latter: lipopeptide composition – for treatment of hypertension, nucleopeptide one – for treatment of hormonal disorders, and phospholipid – for treatment of lung diseases under the surfactant shortage.

The effect of N-stearoylethanolamine on liver phospholipid composition of rats with insulin resistance caused by alimentary obesity

O. V. Onopchenko, G. V. Kosiakova, T. M. Goridko, V. M. Klimashevsky, N. M. Hula

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: onop.89.av@mail.ru

We used alimentary obesity-induced insulin resistance (IR) model in rats to investigate the influence of N-stearoylethanolamine on the content of phospholipids and their fatty acid composition.  Our results show that prolonged high-fat diet triggers considerable aberrations in the composition of main phospholipids in the liver and can be one of the causes of IR in rats. In particular, the increase of phosphatidylcholine, phosphatidylethanolamine and significant decrease of other phospholipids: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine and diphosphaglicerol were observed. The levels of monounsaturated (erucic, nervonic, oleic) and polyunsaturated (eicosatrienoic, docosatrienoic, arachidonic) fatty acids were increased; meanwhile the content of diunsaturated acids was decreased.  The NSE administration (50 mg/kg of body weight) caused restoration of the phospholipids content in the liver of rats with diet-induced IR that highly correlated with the decrease in plasma insulin level and the improvement of insulin sensitivity. Moreover, the effect of NSE was accompanied by the normalization of fatty acids composition of phospholipids that could be related to modulating influen­ce of NSE on the activity of the main fatty acid desaturases.  It is known that the imbalance in phospholipid composition of the rat liver causes substantial metabolic alterations that are associated with the development of IR. Accordingly, the compensations of the imbalance by NSE can help to restore insulin sensitivity, inhibit the development of obesity, IR and type 2 diabetes.

Fatty acid composition of phospholipids and esterified cholesterol of the blood plasma of rabbit under arginine acute pancreatitis

O. O. Hopanenko, J. F. Rivis

Institute for Agriculture Region of Carpathian, National Academy of Agricultural Sciences, Lviv;
e-mail: hopanenko@gmail.com

The content and fatty acid composition of phospholipids and esterified cholesterol were studied in the blood plasma of rabbits under acute arginine pancreatitis and its correction using linseed oil. It is established that the transport and anti-inflammatory functions of blood plasma deteriorates under acute arginine pancreatitis due to a decrease of the content of polyunsaturated fatty acids in phospholi­pids. The amount of cholesterol esterified with saturated and monounsaturated fatty acids increases in the blood plasma of rabbits. The concentration of phospholipids­ and esterified cholesterol is normali­zed and their fatty acid composition is improved in the lipid composition of the blood plasma of rabbits with acute arginine pancreatitis fed with linseed oil.