Ukr.Biochem.J. 2017; Volume 89, Issue 4, Jul-Aug, pp. 43-48

doi: https://doi.org/10.15407/ubj89.04.043

Reversible pH-dependent activation/inactivation of CF(1)-ATPase of spinach chloroplasts

20.07.2017   Uncategorized   No comments

A. P. Khomochkin, O. B. Onoiko, A. V. Semenikhin, O. K. Zolotareva

M. G. Kholodhy Institute of Botany, National Academy of Sciences of Ukraine, Kyiv;
e-mail: membrana@ukr.net

The aim of the work was to study the reverse pH-dependent regulation of the enzymatic activity of the catalytic part of ATP synthase (EC 3.6.3.14) of chloroplast – coupling factor CF1. It was shown that the short-term incubation of isolated CF1 in the media with pH 4.5 or 3.5 leads to inactivation of Ca2+-ATPase, which is rapidly (t1/2 ~ 1 min) restored in the medium containing 0.5-10 mM bicarbonate at pH 7.8. After acid treatment, the rate of Mg2+-ATPase reaction was also stimulated in the presence of 1 mM bicarbonate (рН 7.8; 37 °С). The increase in Ca2+– and Mg2+-АТР activity of CF1 associated with the addition of NaHCO3 solution was completely eliminated after the introduction of 50 mM acetazolamide – a specific inhibitor of carbonic anhydrase. The obtained results suggest the existence of the bound bicarbonate in the CF1 structure, which apparently participates in proton transfer.

Keywords: , , , , ,

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