Ukr.Biochem.J. 2021; Volume 93, Issue 5, Sep-Oct, pp. 122-131
doi: https://doi.org/10.15407/ubj93.05.122
Nobel Prize laureate Kary Mullis and the polymerase chain reaction (PCR)
V. M. Danilova*, O. P. Matyshevska, S. V. Komisarenko
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: valdan@biochem.kiev.ua
Received: 11 May 2021; Accepted: 22 September 2021
The article highlights the major life and career milestones and the extraordinary personality of 1993 Nobel Prize laureate in Chemistry Kary B. Mullis. The background of Mullis’ invention of the polyme-rase chain reaction (PCR), a revolutionary and monumental method of molecular biology and genetics of the 20th century, is described. The PCR technique is based on multiple selective copying of a particular segment of DNA with the help of enzymes in vitro. Under these conditions, only the target region is copied, and only if it is present in the studied sample. The invention of the PCR method has been one of the most outstanding events in molecular biology in recent decades.
Keywords: amplification, DNA, DNA polymerase, history of science, Kary Mullis, polymerase chain reaction (PCR)
References:
- Kleppe K, Ohtsuka E, Kleppe R, Molineux I, Khorana HG. Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA’s as catalyzed by DNA polymerases. J Mol Biol. 1971;56(2):341-361. PubMed, CrossRef
- Matyshevska OP, Danilova VM, Komisarenko SV. The discovery of the mechanisms of biological synthesis of nucleic acids: 1959 Nobel laureates S. Ochoa and A. Kornberg. Ukr Biochem J. 2021;93(1):129-138. CrossRef
- Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230(4732):1350-1354. PubMed, CrossRef
- Regime of access : https://www.nobelprize.org/prizes/chemistry/1993/summary/
- Watson J, Berry A, Davis K. DNA. History of the genetic revolution Chapter 7. The human genome. Life scenario. Regime of access : https://habr.com/ru/company/piter/blog/463873/
- Regime of access : https://uk.wikipedia.org/wiki/Kary Mullis.
- Mullis K. Cosmological significance of time reversal. Nature. 1968;218:663-664. CrossRef
- Molchanova M. Kary Mullis, great and terrible. Chem Life. 2019; (9): 2-5.
- Mullis K. The Polymerase Chain Reaction, Nobel Lecture, December 8, 1993. LEX PRIX NOBEL. The Nobel Prizes. Almqvist and Wiksell Int., Stockholm, Sweden.
- Regime of access : http://www.karymullis.com/pdf/karymullis-cv.pdf
- Wade N. Scientist at Work/Kary Mullis; After the ‘Eureka,’ a Nobelist Drops Out. The New York Times. September 15, 1998.
- Mullis K. Dancing Naked in the Mind Field. USA: Vintage Books, 1998. 240 p.
- Regime of access : https://en.wikipedia.org/wiki/Kary_Mullis
- Volkova NE. Keri Mallis – inventor of PCR (to 70th anniversary). Bull Ukr Soc Genet Breed. 2014;12(1):122-127.
- Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990;262(4):56-61, 64-65. PubMed, CrossRef
- Regime of access : https://studbooks.net/2481604/meditsina/printsip_diagnostiki
- Danylova TV, Komisarenko SV. Standing on the shoulders of giants: James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin and the birth of molecular biology. Ukr Biochem J. 2020;92(4):154-164. CrossRef
- Matyshevska OP, Danilova VM, Komisarenko SV. The discovery of the DNA double helix, or the revolution that ushered in the era of molecular biology (Nobel Prize 1962). Ukr Biochem J. 2020;92(6):183-198. CrossRef
- Chien A, Edgar DB, Trela JM. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol. 1976;127(3):1550-1557. PubMed, PubMedCentral, CrossRef
- Kaledin AS, Sliusarenko AG, Gorodetskii SI. Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1. Biokhimiia. 1980;45(4):644-651. (In Russian). PubMed
- Kary Mullis. Regime of access : https://www.britannica.com/biography/Kary-Mullis
- Regime of access : https://evilleeye.com/wareha….
