Tag Archives: DNA

Studies of the molecular basis of eukaryotic transcription. Roger Kornberg the Nobel prize in Chemistry 2006

O. P. Matyshevska*, V. M. Danylova, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiyv;
*e-mail: matysh@yahoo.com

Received: 18 October 2022; Revised: 25 October 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022

The Nobel Prize in Chemistry 2006 was awarded to an American biochemist and professor of structural biology at Stanford University Roger Kornberg for his fundamental research on the molecular mechanisms of copying genetic information in eukaryotic cells. What are these molecular mechanisms? How is transcription complex formed and what is its structure? R. Kornberg devoted tirelessly 20 years of his work to answer these questions. The article is focused on his research and also describes Roger Kornberg’s life and scientific career.

Oxidative and mutagenic effects of low intensity microwave radiation on quail embryos

A. Burlaka1, O. Tsybulin2*, O. Brieieva1,
O. Salavor3, I. Yakymenko3,4

1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology,
National Academy of Sciences of Ukraine, Kyiv;
2Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine;
3National University of Food Technologies, Kyiv, Ukraine;
4Kyiv Medical University, Kyiv, Ukraine;
*e-mail: alex.tsybulin@gmail.com

Received: 18 December 2020; Accepted: 21 January 2022

Intensive implementation of wireless communication systems raised the question of the possible adverse effects of anthropogenic electromagnetic radiation. This study aims to assess the biological effects of low intensity microwaves (MW) radiation from smartphone Huawei Y5I commercial model used alone or in combination with attached Waveex chip that balances low frequency electromagnetic field but does not affect microwave signal. The biological model of developing quail embryos in ovo was used in the study. The phone as a source of low intensity 1800 MHz (0.32 µW/cm2) microwaves radiation was placed at 3 cm over the surface of hatching eggs and discontinuously activated with a computer program (48 s – on, 12 s – off). It was demonstrated that the exposure of quail embryos to radiation resulted in a statistically significant increase in the content of superoxide, nitrogen oxide and TBA products, DNA integrity damage in embryo cells and increased embryo mortality. Application of Waveex chip during the exposure resulted in a partial normalization of oxidative status and DNA integrity in embryonic cells indicating a negative impact not only of MW radiation, but of low-frequency electromagnetic fields from mobile devices as well.

Breaking the genetic code – a new revolutionary stage in the development of molecular biology: 1968 Nobel Prize laureates M. W. Nirenberg, H. G. Khorana, R. W. Holley

O. P. Matyshevska*, V. M. Danilova, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: matysh@yahoo.com

Received: 28 October 2021; Accepted: 12 November 2021

This review presents the life stories of M. Nirenberg, H. Khorana, and R. Holley, winners of the 1968 Nobel Prize in Physiology or Medicine, the history of the discoveries made by these scientists, and the methodological approaches used in their works. Owing to the M. Nirenberg and H. Khorana research, the nucleotide compositions of all mRNA triplet codons were decoded. H. Khorana was the first scientist to experimentally prove the direct link between the nucleotide sequence of DNA and the amino acid sequence of the synthesized protein and to obtain a synthetic gene. R. Holley was the first to completely decode the sequence of transport RNA, determine its secondary structure and role in protein synthesis on the ribosome. The Nobel Prize awarded to the scientists was a recognition of their contribution in understanding the mechanisms of coding and reading genetic information and marked a breakthrough moment in the development of molecular biology.

Nobel Prize laureate Kary Mullis and the polymerase chain reaction (PCR)

V. M. Danilova*, O. P. Matyshevska, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: valdan@biochem.kiev.ua

Received: 11 May 2021; Accepted: 22 September 2021

The article highlights the major life and career milestones and the extraordinary personality of 1993 Nobel Prize laureate in Chemistry Kary B. Mullis. The background of Mullis’ invention of the polyme-rase chain reaction (PCR), a revolutionary and monumental­ method of molecular biology and genetics of the 20th century, is described. The PCR technique is based on multiple selective copying of a particular segment of DNA with the help of enzymes in vitro. Under these conditions, only the target region is copied, and only if it is present in the studied sample. The invention of the PCR method has been one of the most outstanding events in molecular biology in recent decades.

The discovery of the mechanisms of biological synthesis of nucleic acids: 1959 Nobel laureates S. Ochoa and A. Kornberg

O. P. Matyshevska, V. M. Danilova, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: matysh@yahoo.com

Received: 12 September 2020; Accepted: 17 December 2020

Alongside the chemical and physical research of nucleic acids in the 1940s-50s, the mechanisms of their biosynthesis were investigated. Thus, in 1959, Severo Ochoa and Arthur Kornberg were awarded the Nobel Prize in Physiology or Medicine for the discovery of the mechanisms of biological synthesis of RNA and DNA. The experiments performed by Ochoa and Kornberg are considered today the cornerstone of genetic engineering, as they first demonstrated the possibility of synthesizing RNA and DNA outside the living cell, and also as the enzymes they discovered were among the first tools of this technology.

Standing on the shoulders of giants: James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin and the birth of molecular biology

T. V. Danylova1*, S. V. Komisarenko2

1National University of Life and Environmental Sciences of Ukraine, Kyiv;
*e-mail: danilova_tv@ukr.net;
2Palladin Institute of Biochemistry, National Academy pf Sciences of Ukraine, Kyiv;
e-mail: svk@biochem.kiev.ua

Received: 14 April 2020; Accepted: 15 May 2020

In the 20th century, DNA became a magnet, attracting representatives of various sciences. Prominent researchers competed among themselves to discover the structure of DNA and to explain the mechanisms that determine our “natural fate”, i.e., our heredity. An American chemist, biochemist, chemical engineer Linus Pauling, a British physicist and molecular biologist Maurice Wilkins, a British chemist, biophysicist, and X-ray crystallographer Rosalind Franklin, an American geneticist, molecular biologist, zoologist James Watson, a British molecular biologist, biophysicist, and neuroscientist Francis Crick were among them. They searched for the scientific explanation for the enigma of life hidden in DNA. An accurate description of DNA double-helical structure belongs to James Watson and Francis Crick. However, the missing pieces of the puzzle were elaborated by Rosalind Franklin, who was not given enough credit for her dedicated scientific work. Unlike her, Francis Crick, James Watson, and Maurice Wilkins were awarded the Nobel Prize in Physiology or Medicine 1962 for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material. Whatever the DNA story is, it shows that all great scientific discoveries are not made from scratch. The immense number of people have contributed to the development of science and literally every researcher stands on the shoulders of giants, while the idea itself is in the air. The discovery of the structure of DNA became a cornerstone for the new scientific paradigm – biology acquired a molecular and biochemical basis.

Structural flexibility of canonical 2′-deoxyribonucleotides in DNA-like conformations

T. Yu. Nikolaienko1, L. A. Bulavin1, D. M. Hovorun1,2

1Taras Shevchenko Kyiv National University, Ukraine;
2Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
е-mail: tim_mail@ukr.net

Quantitative characteristics of structural flexibility of the DNA elementary monomer units – 5′-deoxycytidylic, 5′-thymidylic, 5′-deoxyadenylic and 5′-deoxyguanylic acid molecules – have been calculated with original methods. Root-mean-square deviations from equilibrium for all conformational parameters, caused by nuclei thermal or quantum zero-point vibrations, have been found to lie within 4°÷25° at 0 K and 7°÷50° at 298 K and corresponding relaxed force constants – within 1÷35 kcal/mol·rad-2. Their values have been found to be sensitive to the molecule’s conformation. It has been proven, that the torsion angle γ is the most rigid one whereas relaxed force constants for all other conformational variables are lower and comparable to each other. The data obtained could serve for development of structural-dynamical models of the DNA.

The 5′-deoxyadenylic acid molecule conformational capacity: quantum-mechanical investigation using density functional theory (DFT)

T. Yu. Nikolaienko1, L. A. Bulavin1, D. M. Hovorun1,2

1Taras Shevchenko Kyiv National University, Ukraine;
2Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
е-mail: tim_mail@ukr.net

Exhaustive conformational analysis of the 5′-deoxyadenylic acid molecule, has been carried out by the quantum-mechanical density functional theory method at the MP2/6-311++G(d,p) // DFT B3LYP/6-31G(d,p) theory level. As many as 726 of its conformations have been revealed with the relative gas phase Gibbs energies under standard­ conditions from 0 to 12.1 kcal/mole. It has been shown, that the energetically most favorable conformation has north sugar puckering and syn- orien­tation of the nitrogenous base and is stabilized by intramolecular OP1HP1∙∙∙N3 and O3′H∙∙∙OP hydrogen bonds. Four conformations have been shown to have their geometry similar to that of AI-DNA and four – of BI-DNA. One conformer of the 5′-deoxyadenylic acid molecule is similar to its sodium salt hexahydrate structure in crystalline state resolved by the X-ray diffraction method and taken from literature. It is shown that effective charges of C4′ and C5′ atoms are the most sensitive to the molecule conformation ones. The role of the intramolecular OH∙∙∙N hydrogen bonds in formation of the 5′-deoxyadenylic acid molecule structure has been demonstrated.

Conformational variety and physical properties of the 1,2-dideoxyribofuranose-5-phosphate, the model DNA monomer structural unit

T. Yu. Nikolaienko1, L. A. Bulavin1, D. M. Hovorun1,2, O. O. Missura1

1Taras Shevchenko Kyiv National University, Ukraine;
2Institute of Molecular Biology and Genetics,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: tim_mail@ukr.net

The results of exhaustive quantum-mechani­cal conformational analysis of 1,2-dideoxyribofuranose-5-phosphate molecule, the model DNA backbone structural unit, are presented. As many as 282 conformations with the relative Gibbs energies from 0 to 8.9 kcal/mole have been obtained at the MP2/cc-pVTZ // DFT B3LYP/cc-pVTZ theory level. Among them seven structures are similar to those of the DNA backbone in its AI, BI and ZII forms, while the B-DNA-like conformation has the lowest Gibbs energy (ΔG = 3.3 kcal/mole). It is shown that the relaxed force constants values­ for conformational parameters of all DNA-like conformations satisfy inequality Kγ > Kα′ > Kε > Kβ.

Estimation of the ROS in the presence of biologically active substances by porous silicon fluorescence

V. B. Shevchenko1, O. I. Dacenko1, O. V. Shablykin2, T. V. Osadchuk2,
A. M. Lyakhov2, Y. V. Pivovarenko3, V. A. Makara1,3

1Taras Shevchenko Kyiv National University, Ukraine;
e-mail: shevchenko@univ.kiev.ua;
2Institute of Bioorganic Chemistry and Petroleum Chemistry,
National Academy of Sciences of Ukraine;
3STC Physico-Chemical Center of Materials Science
of Taras Shevchenko Kyiv National University and NAS of Ukraine

The fluorescence spectra of the porous silicon modified by water solutions of biologically active materials and materials of biological origin are recorded as well as the fluorescence spectra of the porous silicon modified by lecithin monolayers grown on the surface of water solutions of the biologically active materials. The analysis of the obtained spectra made it possible to conclude on the effect of the studied materials on the content of ROS.