Ukr.Biochem.J. 2023; Volume 95, Issue 6, Nov-Dec, pp. 64-72

doi: https://doi.org/10.15407/ubj95.06.064

AUT-M enterosorbent stabilizes glutathione system in vincristine-treated rats with dimethylhydrazine-induced colon cancer

11.12.2023   Uncategorized   No comments

O. I. Kachur*, L. S. Fira, P. H. Lykhatskyі,
I. R. Bekus, M. V. Kyryliv

I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: oksana.kachur2012@gmail.com

Received: 15 August 2023; Revised: 17 October 2023;
Accepted: 01 December 2023; Available on-line: 18 December 2023

Colorectal cancer is one of the leading causes of mortality in the world. The search for new methods of therapy for this disease that could correct the state of oxidative stress during the development of neoplasms is up to date. The aim of this work was to study the level of reduced glutathione and the activity of glutathione-dependent enzymes in the development of 1,2 dimethylhydrazine-induced colon cancer in rats while treated with vincristine and the use of enterosorbent. To induce carcinogenesis, dimethylhydrazine was administered to male rats subcutaneously for 30 weeks at a dose of 7.2 mg/kg of body weight. The rats with induced colon cancer received entorosorbent per os at a dose of 0.2 g per 100 g of body weight daily for 21 days. After detoxification therapy, the rats were administered cytostatic vincristine daily at a dose of 0.23 mg/kg for 14 days. A decrease in the content of reduced glutathione, the activity of glutathione reductase and glutathione peroxidase in the blood and liver tissue of rats with colorectal cancer was established. The use of enterosorbent­ AUT-M was shown to be effective in stabilizing the indicators of the glutathione system in rats with induced colon cancer. Cytostatic vincristine did not significantly affect the change of the studied indicators, confirming the effectiveness of previous sorption measures.

Keywords: , , , , , , ,

References:

  1. Marushchak M, Maksiv K, Krynytska I, Stechyshyn I. Glutathione antioxidant system of lymphocytes in the blood of patients in a setting of concomitant chronic obstructive pulmonary disease and arterial hypertension. Pol Merkur Lekarski. 2019;47(281):177-182. PubMed
  2. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017:2017:8416763. PubMed, PubMedCentral, CrossRef
  3. Posokhova K, Stechyshyn I, Krynytska I, Marushchak M, Birchenko I, Klishch I. Comparative study of the effect of various forms of quercetin on experimental diabetes. Rom J Diabetes Nutr Metab Dis. 2018;25(4):383–388. CrossRef
  4. Bansal A, Simon MC. Glutathione metabolism in cancer progression and treatment resistance. J Cell Biol. 2018;217(7):2291-2298. PubMed, PubMedCentral, CrossRef
  5. Jiao Y, Wang Y, Guo S, Wang G. Glutathione peroxidases as oncotargets. Oncotarget. 2017;8(45):80093-80102. PubMed, PubMedCentral, CrossRef
  6. Kennedy L, Sandhu JK, Harper ME, Cuperlovic-Culf M. Role of Glutathione in Cancer: From Mechanisms to Therapies. Biomolecules. 2020;10(10):1429. PubMed, PubMedCentral, CrossRef
  7. Minich DM, Brown BI. A Review of Dietary (Phyto)Nutrients for Glutathione Support. Nutrients. 2019;11(9):2073. PubMed, PubMedCentral, CrossRef
  8. Shevchuk O, Snezhkova E, Sarnatskaya V, Mikhailenko V, Glavin A, Makovetska L, Bardakhivska K, Birchenko I, Kozynchenko O, Nikolaev V. Effect of Primary and Secondary Beads of Carbon Enterosorbent on Haematological Parameters and Oxidative Stress Development Caused by Melphalan in Rats. Medicina (Kaunas). 2019 Sep 2;55(9):557. PubMed, PubMedCentral, CrossRef
  9. Sakhno LA, Babenko LP, Lazarenko LM, Korotych VG, Sarnatskaya VV, Snezhkova EA, Spivak MY, Nikolaev VG. Efficacy of dispersed fibrous carbon sorbent in treatment of malignant fungating wounds. Exp Oncol. 2021;43(4):359-364. PubMed, CrossRef
  10. Sakhno LA, Sarnatskaya VV, Yushko LA, Snezhkova EA, Bardakhivskaya KI, Shevchuk OO, Sidorenko AS, Nikolaev VG. Adsorptive therapy as a modificator for tumor-host interaction. Exp Oncol. 2019;41(3):254-257. PubMed, CrossRef
  11. Sarnatskaya V, Shlapa Y, Lykhova A, Brieieva O, Prokopenko I, Sidorenko A, Solopan S, Kolesnik D, Belous A, Nikolaev V. Structure and biological activity of particles produced from highly activated carbon adsorbent. Heliyon. 2022;8(3):e09163. PubMed, PubMedCentral, CrossRef
  12. Soroka Y, Andriichuk I, Lykhatskyi P, Fira L, Lisnychuk N. Violation of the prooxidant-antioxidant balance in the spleen tissue under experimental carcinogenesis. Georgian Med News. 2020;(308):123-128. (In Russian). PubMed
  13. Nair A, Morsy MA, Jacob S. Dose translation between laboratory animals and human in preclinical and clinical phases of drug development. Drug Dev Res. 2018;79(8):373-382. PubMed, CrossRef
  14. Vlizlo VV, Fedoruk RS. Laboratory methods of investigation in biology, stock- breeding and veterinary. Reference book. Spolom, Lviv: 2012. 764.
  15. Bardelčíková A, Šoltys J, Mojžiš J. Oxidative Stress, Inflammation and Colorectal Cancer: An Overview. Antioxidants (Basel). 2023;12(4):901. PubMed, PubMedCentral, CrossRef
  16. Gerashchenko BI, Sarnatskaya VV, Bardakhivska KI, Sydorenko OS, Kolesnik DL, Klymchuk DO. Myeloprotection with activated carbon in doxorubicin-treated rats. Heliyon. 2023;9(7):e18414. PubMed, PubMedCentral, CrossRef
  17.  Gunasekaran S, Venkatachalam K, Namasivayam N. Anti-inflammatory and anticancer effects of p-methoxycinnamic acid, an active phenylpropanoid, against 1,2-dimethylhydrazine-induced rat colon carcinogenesis. Mol Cell Biochem. 2019;451(1-2):117-129. PubMed, CrossRef
  18. Kachur О, Fira L, Lykhatskyy P, Fira D, Stechyshyn I. The state of pro-and antioxidant systems in rats with DMH-induced colon carcinogenesis on the background of extracorporeal detoxification. Pharmacia. 2021;68:941. CrossRef
  19. Koeberle SC, Gollowitzer A, Laoukili J, Kranenburg O, Werz O, Koeberle A, Kipp AP. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-κB-driven inflammation through redox-active mechanisms. Redox Biol. 2020.28:101388. PubMed, PubMedCentral, CrossRef
  20. Kachur O, Fira L, Lykhatskyі P, Fira D, Garlitska N. Evaluation of membrane-destructive processes in rats with induced carcinogenesis of the colon using the cytostatic vincristine. Rocz Panstw Zakl Hig. 2022;73(2):215-220. PubMed, CrossRef
  21. Hassan AK, El-Kalaawy AM, Abd El-Twab SM, Alblihed MA, Ahmed OM. Hesperetin and Capecitabine Abate 1,2 Dimethylhydrazine-Induced Colon Carcinogenesis in Wistar Rats via Suppressing Oxidative Stress and Enhancing Antioxidant, Anti-Inflammatory and Apoptotic Actions. Life (Basel). 2023;13(4):984. PubMed, PubMedCentral, CrossRef
  22. Mandal P. Potential biomarkers associated with oxidative stress for risk assessment of colorectal cancer. Naunyn Schmiedebergs Arch Pharmacol. 2017;390(6):557-565. PubMed, CrossRef
  23. Morsy HM, Ahmed OM , Zoheir KMA, Abdel-Moneim A. The anticarcinogenic effect of eugenol on lung cancer induced by diethylnitrosamine/2-acetylaminofluorene in Wistar rats: insight on the mechanisms of action. Apoptosis. 2023;28(7-8):1184-1197. PubMed, PubMedCentral, CrossRef
  24. Mys LA, Strutynska NA, Goshovska YV, Sagach VF. Stimulation of the endogenous hydrogen sulfide synthesis suppresses oxidative-nitrosative stress and restores endothelial-dependent vasorelaxation in old rats. Can J Physiol Pharmacol. 2020;98(5):275-281. PubMed, CrossRef
  25. Škubník J, Svobodová Pavlíčková V, Ruml T, Rimpelová S. Vincristine in Combination Therapy of Cancer: Emerging Trends in Clinics. Biology (Basel). 2021;10(9):849. PubMed, PubMedCentral, CrossRef
  26. Madsen ML, Due H, Ejskjer N, Jensen P, Madsen J, Dybker K. Aspects of vincristine-induced neuropathy in hematologic malignancies: a systematic review. Cancer Chemother Pharmacol. 2019;84(3):471-485. PubMed, PubMedCentral, CrossRef
  27. Saeedan AS, Rastogi S, Ansari MN. Roflumilast counteracts DMH-induced preneoplastic colon damage in albino Wistar rats. Hum Exp Toxicol. 2020;39(11):1545-1555. PubMed, CrossRef
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