Tag Archives: glutathione reductase
AUT-M enterosorbent stabilizes glutathione system in vincristine-treated rats with dimethylhydrazine-induced colon cancer
O. I. Kachur*, L. S. Fira, P. H. Lykhatskyі,
I. R. Bekus, M. V. Kyryliv
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine;
*e-mail: oksana.kachur2012@gmail.com
Received: 15 August 2023; Revised: 17 October 2023;
Accepted: 01 December 2023; Available on-line: 18 December 2023
Colorectal cancer is one of the leading causes of mortality in the world. The search for new methods of therapy for this disease that could correct the state of oxidative stress during the development of neoplasms is up to date. The aim of this work was to study the level of reduced glutathione and the activity of glutathione-dependent enzymes in the development of 1,2 dimethylhydrazine-induced colon cancer in rats while treated with vincristine and the use of enterosorbent. To induce carcinogenesis, dimethylhydrazine was administered to male rats subcutaneously for 30 weeks at a dose of 7.2 mg/kg of body weight. The rats with induced colon cancer received entorosorbent per os at a dose of 0.2 g per 100 g of body weight daily for 21 days. After detoxification therapy, the rats were administered cytostatic vincristine daily at a dose of 0.23 mg/kg for 14 days. A decrease in the content of reduced glutathione, the activity of glutathione reductase and glutathione peroxidase in the blood and liver tissue of rats with colorectal cancer was established. The use of enterosorbent AUT-M was shown to be effective in stabilizing the indicators of the glutathione system in rats with induced colon cancer. Cytostatic vincristine did not significantly affect the change of the studied indicators, confirming the effectiveness of previous sorption measures.
Intensity of peroxidation processes and activity of antioxidant enzymes in rat tissues at high chromium level in the diet
R. Ya. Iskra, V. G. Yanovych
Institute of Animal Biology, National Academy of Agrarian Sciences, Lviv, Ukraine;
e-mail: ruslana_iskra@inenbiol.com.ua
The data on the influence of chromium in different tissues of rats at its consumption with mixed fodder in the form of CrCl3×6H2O on the intensity of peroxidation processes and activity of antioxidant enzymes are presented. The degree of high chromium content in the studied tissues of rats at its addition to mixed fodder in the amount of 200 µg/kg during 30 days was established. Chromium content in the rat tissues decreased in the order: the spleen, heart, kidneys, lungs, brain, liver, skeletal muscle. In all tissues of rats fed with mixed fodder with chromium addition, except for skeletal muscles, content of lipid peroxidation products – hydroperoxide and TBARS-products decreased. The content of lipid peroxidation products decreased in the spleen, kidneys, liver and lungs. Also in all organs and tissues of rats the activity of glutathione peroxidase, glutathione reductase and catalase increased at the action of chromium. In the brain and kidneys the level of reduced glutathione increased. Superoxide dismutase activity was significantly higher not only in the heart and skeletal muscles of animals and is probably equal in the lungs and liver, and in other organs – the brain, kidneys and spleen in animals of the studied group the enzyme activity was lower as compared to animals of the control group. Obtained results demonstrate the regulatory influence of chromium on free radical process in the rat tissues.
Ascorbate-glutathione cycle enzymes activity in zea mays leaves under salinity and treatment by adaptogenic compounds
O. O. Konturska, T. O. Palladina
M. G. Kholodny Institute of Botany, National Academy of Science of Ukraine, Kyiv;
e-mail: konturska@ukr.net
The effect of different salinity levels and synthetic compounds treatments on ascorbate-glutathione cycle enzymes activity in maize leaves has been investigated. One-day seedlings exposition with 0.05 М NaCl increased ascorbate peroxidase activity, whereas 10-day exposition did not affect it. However the exposition with 0.1 M NaCl, which is extreme for maize, decreased ascorbate peroxidase activity in leaves during 10 days. On the other hand glutathione reductase activity in leaves increased under both salt concentrations. Seeds treatments with Methyure and Ivine increased ascorbate peroxidase activity in the leaves of seedlings under 0.1 М NaCl, but did not affect glutathione reductase activity as compared to the salt control. The results obtained have shown differences of ascorbate-glutathione cycle enzymes responses to salt exposition of seedlings and the effects of adaptogenic compounds on the ascorbate-glutathione cycle via ascorbate peroxidase activation.
Activity of the glutathione system of antioxidant defense in rats under the action of L-glutamic acid
N. O. Salyha
Institute of Animal Biology, National Academy of Agrarian Sciences of Ukraine, Lviv, Ukraine;
e-mail: ynosyt@yahoo.com
The data on the effects of glutamic acid (L-Glu), which is one of three amino acid – precursors of glutathione on animals organism is quite controversial because research in this area remain relevant. The aim of our research was to find out what impact the additional introduction of L-Glu on the activity glutathione system of antioxidant defence and the content of lipid peroxidation products in various organs and tissues of rats. The effect of additional (285 and 715 mg/kg, respectively) introduction to the diet of L-Glu on the activity of antioxidant enzymes and intensity of peroxidation processes in various tissues of rats was studied. It is shown that in the liver, spleen and kidneys of rats which received additional 715 mg/kg of L-Glu content of reduced glutathione and glutathione peroxidase activity increased. A decrease of the content of lipid hydroperoxides and TBA-active products in tissues of animals which received additional 285 and 715 mg/kg of L-Glu into the diet was found. We have also found that the enrichment of rat’s diet by L-Glu during 30 days resulted in a change of glutathione part of antioxidant system and intensity of lipid peroxidation. More intensive changes in these indices were observed in animals which received additional 715 mg/kg of L-Glu into the diet.
Changes in glutathione system and lipid peroxidation in rat blood during the first hour after chlorpyrifos exposure
V. P. Rosalovsky, S. V. Grabovska, Yu. T. Salyha
Institute of Animal Biology, National Academy of Agrarian Sciences of Ukraine, Lviv;
e-mail: ros.volodymyr@gmail.com
Chlorpyrifos (CPF) is a highly toxic organophosphate compound, widely used as an active substance of many insecticides. Along with the anticholinesterase action, CPF may affect other biochemical mechanisms, particularly through disrupting pro- and antioxidant balance and inducing free-radical oxidative stress. Origins and occurrence of these phenomena are still not fully understood. The aim of our work was to investigate the effects of chlorpyrifos on key parameters of glutathione system and on lipid peroxidation in rat blood in the time dynamics during one hour after exposure. We found that a single exposure to 50 mg/kg chlorpyrifos caused a linear decrease in butyryl cholinesterase activity, increased activity of glutathione peroxidase and glutathione reductase, alterations in the levels of glutathione, TBA-active products and lipid hydroperoxides during 1 hour after poisoning. The most significant changes in studied parameters were detected at the 15-30th minutes after chlorpyrifos exposure.