Tag Archives: actomyosin
Influence of variuos regimens ultrasaund on oxide-modified actomyosin superprecipitation reaction from skeletal muscle of rabbit
O. V. Shelyuk, N. Ye. Nurishchenko, K. O. Medynska
Taras Shevchenko Kyiv National University, Ukraine;
e-mail: shelyuk_olga@ukr.net
A comparative study of rabbit skeletal muscles oxide-modified actomyosin superprecipitation reaction in dependence on continuous and impulsive (2 ms) ultrasound regimens was studied. From the analyses of kinetic curves the effect of the value of superprecipitation (Аm – А0), time t1/2, required for achievement of half of its value was determined, and the normalized maximal rate of this reaction Vn was also calculated. It is shown that the use of continuous ultrasound to oxide-modified actomyosin was associated with a significant decrease of superprecipitation relative to controls. However, pulsed ultrasound caused a significant increase in superprecipitation value except for the values (Аm – А0) in the application of the intensity of 0.2 W/cm2. The oxide-modified actomyosin superprecipitation value under the effect of continuous and impulsive ultrasound at intensity 1 W/cm2 in relative to control and all other applied intensities decrease to the most extent. It is caused perhaps by thermal influence of ultrasound. In general, the data obtained give reason to assume that the effects of continuous and pulsed ultrasound on the reaction of oxide-modified proteins complex superpretsipitatsii identical.
Effect of electromagnetic field of extremely low frequency on ATPase activity of actomyosin
Yu. V. Tseyslyer, O. V. Shelyuk, V. S. Martynyuk, N. E. Nuryschenko
ESC Institute of Biology, Taras Shevchenko Kyiv National University, Ukraine;
e-mail: yuliya.tseysler@gmail.com;
shelyuk_olga@ukr.net
The Mg2+/Ca2+ and K+-ATPase actomyosin activity of rabbit skeletal muscle was evaluated by the Fiske-Subbarow method during a five-hour exposition of protein solutions in electromagnetic field of extremely low frequency of 8 Hz and 25 µT induction. The results of the study of the ATPase activity of actomyosin upon electromagnetic exposure have shown statistically significant changes that are characterized by a rather complex time dynamics. After 1, 2 and 4 hours of exposure of protein solutions the effect of ELF EMF exposure inhibits the ATPase activity compared to control samples, which are not exposed to the magnetic field. By the third and fifth hours of exposure to the electromagnetic field, there is a significant increase in the ATPase activity of actomyosin. It should be noted that a similar pattern of change in enzyme activity was universal, both for the environment by Mg2+ and Ca2+, and in the absence of these ions in the buffer. This can evidence for Ca2+-independent ways of the infuence of electromagnetic field (EMP) on biologic objects. In our opinion, the above effects are explained by EMP influence on the dynamic properties of actomyosin solutions, which are based on the processes of spontaneous dynamic formation of structure.