Tag Archives: bile acids
Long-term hypocholesterolemic effect of amidated alginate in rats
M. Marounek1, Z. Volek1, T. Taubner1, D. Dušková1, L. Kalachniuk2
1Institute of Animal Science, Prague, Czech Republic;
e-mail: marounek.milan@vuzv.cz;
2National University of Life and Environmental Sciences of Ukraine, Kyiv;
e-mail: kalachnyuk_liliya@nubip.edu.ua; lilkalachnyuk@gmail.com
The effect of octadecylamide of alginic acid on blood serum and hepatic cholesterol, and the faecal output of fat and sterols was examined in female rats fed diets containing cholesterol and palm fat at 10 and 50 g/kg, respectively for 10 weeks. Amidated alginate, supplied at 10 and 20 g/kg, significantly decreased serum cholesterol from 5.25 to 2.99 and 2.39 µmol/ml, respectively, and decreased hepatic cholesterol from 30.7 to 12.3 and 9.4 µmol/g, respectively. Amidated alginate increased the faecal output of fat and at higher dosing significantly decreased faecal output of bile acids. Faecal output of bile acids and hepatic cholesterol significantly correlated (r = 0.791; P < 0.001). The results of the present experiment showed that hypocholesterolemic effect of amidated alginate persisted within 10 weeks of feeding.
Correlations of bile acids in the bile of rats in conditions of alloxan induced diabetes melitus
N. N. Danchenko, S. P. Veselskiy, B. А. Tsydzevich
Taras Shevchenko National University of Kyiv, ESC Institute of Biology, Ukraine;
e-mail: ninadanc@gmail.com
The ratio of bile acids in the bile of rats with alloxan diabetes was investigated using the method of thin-layer chromatography. Changes of coefficients of conjugation and hydroxylation of bile acids were calculated and analyzed in half-hour samples of bile obtained during the 3-hour experiment. It has been found that the processes of conjugation of cholic acid with glycine and taurine are inhibited in alloxan diabetes. At the same time a significant increase of free threehydroxycholic and dіxydroxycholic bile acids and conjugates of the latter ones with taurine has been registered. Coefficients of hydroxylation іn alloxan diabetes show the domination of “acidic” pathway in bile acid biosynthesis that is tightly connected with the activity of mitochondrial enzymes.