Tag Archives: bilirubin

ТiО(2) hepatotoxicity under long-term administration to rats

O. V. Tsymbalyuk, S. P. Veselsky, A. M. Naumenko, T. L. Davydovska,
I. S. Voiteshenko, I. I. Сhyzh, V. A. Skryshevsky

Institute of High Technologies, Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: otsymbal@bigmir.net

Received: 30 March 2020; Accepted: 15 May 2020

Titanium dioxide (ТіО2) powder which is used as a white dye was  considered to be an inert material for a long time despite its accumulation  in liver tissues after penetration into  organism. The aim of the study was to estimate biochemical markers of liver functioning in blood plasma and ATPase activity of erythrocyte plasma membrane under the oral administration of ТіО2 nanoparticles suspension (0.1 mg/kg, daily) to Wistar rats for 30 and 100 days. A significant increase of alanine aminotransferase and aspartate aminotransferase activity as well as of direct, indirect and bound bilirubin content, a decrease of connjugated (taurocholic, taurochenodeoxycholic, taurodeoxycholic, glycocholic, glycochenodeoxycholic, and glycodeoxycholic) and free (glycodeoxycholic and deoxycholic) bile acids concentration with concomitant increase of free cholic acid concentration in blood plasma of rats under ТіО2 administration were revealed, indicating a significant impairment of pigment exchange in the liver of rats. Under ТіО2 administration a substantial  inhibition of erythrocyte plasma membrane Мg2+-dependent ouabain-sensitive Na+,K+-ATPase and ouabain-insensitive ATPase was observed. These results presume the disturbance of transplasmalema ion-transporting processes and cells ionic homeostasis  induced by ТіО2.

Alpha-fetoprotein as a biochemical diagnostic and prognostic marker for prolonged jaundice in newborns

O. H. Mazur, O. S. Yablon, O. S. Rubina,
M. M. Puhach, A. P. Konoplitska

National Pirogov Memorial Medical University, Vinnytsia, Ukraine;
e-mail: alena523@ukr.net

Received: 14 January 2019; Accepted: 13 August 2019

Prolonged jaundice of newborns is a common pathology during the neonatal period. Recently, there has been a tendency toward an increased number of newborns with prolonged jaundice with duration longer than 14 days of life. According to the polyetiologic nature of neonatal jaundice, it is necessary to investigate new diagnostic signs that can predict the development of prolonged jaundice in newborns and, allow identification of new methods of differential diagnosis of neonatal jaundice, as well as decrease the frequency of this pathology. The parameters of serum alpha-fetoprotein (AFP) content in newborns with prolonged jaundice were studied. The content of AFP in blood serum was 1.7 times higher in newborns with prolonged jaundice than in newborns with jaundice for up to 14 days of life: Me = 671.1 [Q1 = 479.9; Q3 = 815.03] ng/ml and Me = 401.0 [Q1 = 284.9; Q3 = 684.0] ng/ml, respectively (P < 0.05). The content of blood serum AFP in newborns with prolonged jaundice was higher for total serum bilirubin greater than 250 μmole/l: Me = 626.2 [Q1 = 454.7; Q3 = 793.2] ng/ml which was confirmed by a strong direct correlation relationship (rxy = 0.64, P < 0.05). The results of the ROC analysis confirmed that AFP ≥ 571.7 ng/ml can identify newborns with prolonged jaundice with a sensitivity of 74.2% and a specificity of 74.5%. The area under the curve (AUC) was 0.870 (95% CI 0.804, 0.937), which confirms the good quality of the AFP model ≥ 571.7 ng/ml.