Tag Archives: correlation analysis
Fetuin-A level in patients with untreated thyroid dysfunction
A. S. Idan1*, M. I. Hamzah1, M. S. Khudhair2
1Clinical Biochemistry Department, Al-Nahrain University, Medicine College, Iraq;
2Internal Medicine Department, Al-Nahrain University, Medicine College, Iraq;
*e-mail: alaas3223@gmail.com
Received: 26 August 2023; Revised: 08 November 2023;
Accepted: 01 February 2024; Available on-line: 26 February 2024
Fetuin-A, a plasma glycoprotein, has been demonstrated to play an essential role in the pathogenesis of several metabolic disorders. This study aimed to estimate fetuin-A serum level in patients with newly diagnosed primary hyperthyroidism (PHT) and subclinical hypothyroidism (SCH) and to examine its correlation with thyroid hormones level, age and sex of patients. The study involved 90 patients with untreated thyroid dysfunction verified with thyroid function test (45 with PHT and 45 with SCH) and 90 control subjects. Triiodothyronin (T3), tetraiodothyronin (T4), and thyroid stimulating hormone (TSH) serum concentrations were measured with enzyme-linked fluorescent assay (ELFA), fetuin-A concentration was measured with enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the level of fetuin-A was significantly higher in the PHT group as compared with the control group and showed a significant positive correlation with the T3 level. In the SCH group, the level of fetuin-A was significantly lower and showed a negative correlation with TSH level. Fetuin-A level rose with age in the PHT group and was unaffected by sex in all studied groups. The perfect AUC value obtained for fetuin-A in the comparison between PHT and SCH groups suggests its potential use as a reliable diagnostic marker to differentiate between these two thyroid conditions.
Indoleamine 2,3-dioxygenase level and oxidative stress parameters in the serum of patients with chronic renal failure
F. M. Y. Saeed, R. F. Jasim*
College of Education for Girls, Department of Chemistry, University of Mosul, Iraq;
*e-mail: ra.fadhel@uomosul.edu.iq
Received: 13 April 2023; Revised: 02 June 2023;
Accepted: 07 September 2023; Available on-line: 12 September 2023
Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-degrading enzyme belonging to the kynurenine pathway. IDO activity has been suggested as a biomarker for diagnosis of chronic kidney disease. The aim of the study was to estimate the level of IDO, urea, creatinine, uric acid, phosphate, calcium, albumin, MDA, GSH, and activity of peroxidase, catalase, arylesterase in the serum of chronic renal failure (CRF) patients treated with dialysis compared to the healthy control group. The results showed a significant increment in IDO level in patients compared with the control. Linear regression analysis using the Pearson correlation coefficient showed that increased IDO level correlates positively with urea, creatinine, uric acid, phosphate, MDA level and peroxidase activity whereas negatively with albumin, calcium, glutathione level, catalase activity and glomerular filtration rate. We concluded that IDO level might be a possible marker of oxidative stress and inflammation in patients with CRF.
Fatty acid composition of sulfate-reducing bacteria isolated from technogenic ecotopes
D. R. Аbdulinа, G. O. Iutynska, L. M. Purish
Danylo Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: adara@ukr.net
Received: 14 June 2019; Accepted: 15 May 2020
The growth of technogenic (man-caused) load on the environment leads to the disturbance of natural ecotopes and is a stress factor for the widespread sulfate-reducing bacteria (SRB). Changes of SRB fatty acid composition are considered to be not only one of the mechanisms of adaptation and protection from negative stress but also one of the chemotaxonomic features that can be used as the indicator of bacteria genus and its presence in natural ecotopes. The aim of the work was to determine the fatty acid composition of sulfate-reducing bacteria strains isolated from different technogenic ecotopes. The spectrum of 17 fatty acids was determined by gas chromatography-mass spectrometry. The predominance of saturated C14:0, C15:0, C16:0 and C18:0 and the presence of unsaturated C16:1 and C18:1 fatty acids in SRB lipids were demonstrated. Correlation analysis showed that SRB isolated from the same technogenic locations were characterized by substantial similarity of fatty acid profiles despite belonging to different genera. Thus, fatty acid compositions of SRB strains Desulfovibrio sp. K1 and K2 isolated from soils near gas main-pipeline had correlation index r = 0.94 and that Desulfovibrio sp. TC2, Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 isolated from city heat system ecotope had correlation index r = 0.97-0.99. The obtained data on increased saturation degree of SRB fatty acids and decreased membrane fluidity indexes could be used for assessing the degree of SRB adaptation to the influence of man-caused loading as a stress factor.