Ukr.Biochem.J. 2020; Volume 92, Issue 4, Jul-Aug, pp. 103-110

doi: https://doi.org/10.15407/ubj92.04.103

Fatty acid composition of sulfate-reducing bacteria isolated from technogenic ecotopes

07.09.2020   Uncategorized   No comments

D. R. Аbdulinа, G. O. Iutynska, L. M. Purish

Danylo Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: adara@ukr.net

Received: 14 June 2019; Accepted: 15 May 2020

The growth of technogenic (man-caused) load on the environment leads to the disturbance of natural ecotopes and is a stress factor for the widespread sulfate-reducing bacteria (SRB). Changes of SRB fatty acid composition are considered to be not only one of the mechanisms of adaptation and protection from negative stress but also one of the chemotaxonomic features that can be used as the indicator of bacteria genus and its presence in natural ecotopes. The aim of the work was to determine the  fatty acid composition of sulfate-reducing bacteria  strains isolated from different technogenic ecotopes. The spectrum of 17 fatty acids was determined by gas chromatography-mass spectrometry. The predominance of saturated C14:0, C15:0, C16:0 and C18:0 and the presence of unsaturated C16:1 and C18:1 fatty acids in SRB lipids were demonstrated. Correlation analysis showed that SRB isolated from the same technogenic locations were characterized by substantial similarity of fatty acid profiles despite belonging to different genera. Thus, fatty acid compositions of SRB strains Desulfovibrio sp. K1 and K2 isolated from soils near gas main-pipeline had correlation index r = 0.94 and that Desulfovibrio sp. TC2, Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 isolated from city heat system ecotope had correlation index r = 0.97-0.99. The obtained data on increased saturation degree of SRB fatty acids and decreased membrane fluidity indexes could be used for assessing the degree of SRB adaptation to the influence of man-caused loading as a stress factor.

Keywords: , , ,

References:

  1. Andreyuk KI, Kozlova IP, Kopteva ZhP, Pilyashenko-Novokhatny AI, Zanina VV, Purish LM. Microbial corrosion of underground constructions. Kyiv: Naukova Dumka, 2005. 260 p. (In Ukrainian).
  2. Johnson MS, Zhulin IB, Gapuzan ME, Taylor BL. Oxygen dependent growth of the obligate anaerobe Desulfovibrio vulgaris Hildenborough. J Bacteriol. 1997;179(17):5598-5601. PubMed, PubMedCentral, CrossRef
  3. Baysse C, O’Gara F. Role of membrane structure during stress signalling and adaptation in Pseudomonas. Pseudomonas. 2007;7:193-224. CrossRef
  4. Ueki A, Suto T. Cellular fatty acid composition of sulfate-reducing bacteria. J Gen Appl Microbiol. 1979; 25(3):185-196. CrossRef
  5. Vainshtein MB, Gogotova GI, Galushko AS. Grouping of sulfate-reducing bacteria by spectral properties of cytochrome c. Mikrobiologiya. 1996; 65(2): 160-164. (In Russian).
  6. Аsaulenko LG, Аbdulina DR, Purish LM. Taxonomic position of certain representatives of sulphate-reducing corrosive microbial community. Mikrobiol Zhurn. 2010;72(4):3-10. (In Ukrainian). PubMed
  7. Abdulina DR, Purish LM, Iutynska GO. Microbial communities and sulphate-reducing bacteria in soils near main gas-pipeline. Mikrobiol Zhurn. 2018; 80(5): 3-14. CrossRef
  8. Purish LM, Asaulenko LG, Abdulina DR, Iutinskaia GA. Biodiversity of sulfate-reducing bacteria growing on objects of heating systems. Mikrobiol Zhurn. 2014;76(3):11-17. (In Russian). PubMed
  9. Varbanets LD, Zdorovenko GM, Knirel YuA. Methods of endotoxins investigation. К.: Naukova Dumka, 2006. 238 p. (In Russian).
  10. Guerzoni ME, Lanciotti R, Cocconcelli PS. Alteration in cellular fatty acid composition as a response to salt, acid, oxidative and thermal stresses in Lactobacillus helveticus. Microbiology. 2001;147(Pt 8):2255-2264. PubMed, CrossRef
  11. Härtig C, Loffhagen N, Harms H. Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida. Appl Environ Microbiol. 2005;71(4):1915-1922. PubMed, PubMedCentral, CrossRef
  12. Kupalova HI. The theory of economic analysis. Mannual. Kyiv: 2008. 639 p. (In Ukrainian).
  13. Denich TJ, Beaudette LA, Lee H, Trevors JT. Effect of selected environmental and physico-chemical factors on bacterial cytoplasmic membranes. J Microbiol Methods. 2003;52(2):149-182. PubMed, CrossRef
  14. Duldhardt I, Gaebel J, Chrzanowski L, Nijenhuis I, Härtig C, Schauer F, Heipieper HJ. Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition. Microbial Biotechnol. 2010;3(2):201-209. PubMed, PubMedCentral, CrossRef
  15. Murínová S, Dercová K. Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane. Int J Microbiol. 2014;2014: 1-16.  PubMed, PubMedCentral, CrossRef
  16. 16. Boon JJ, de Leeuw JW, Hoek GJ, Vosjan JH. Significance and taxonomic value of iso and anteiso monoenoic fatty acids and branched β-hydroxy acids in Desulfovibrio desulfuricans. J Bacteriol. 1977;129(3):1183–1191. PubMed, PubMedCentral, CrossRef
  17. Nazina TN, Turova TP, Ivanova AE, Belyaev SS, Poltaraus AB, Gryadunov DA, Osipov GA. Phylogenetic position and chemotaxonomic characteristics of the thermophilic sulfate-reducing bacterium Desulfotomaculum kuznetsovii.  Mikrobiologiya. 1999; 68(1): 92-99. (In Russian).
  18. Fichtel K, Logemann J, Fichtel J, Rullkötter J, Cypionka H, Engelen B. Temperature and pressure adaptation of a sulfate reducer from the deep subsurface. Front Microbiol. 2015;6:10781. PubMed, PubMedCentral, CrossRef
  19. Markowicz A, Plociniczak T, Piotrowska-Seget Z. Response of bacteria to heavy metals measured as changes in FAME profiles. Polish J Environ Stud. 2010;19(5):957-965.
  20. Maslovska O, Hnatush S, Halushka A. Fatty acids composition of Desulfuromonas acetoxidans IMV B-7384 cells under the influence of ferric citrate. Studia Biologica. 2014;8(3-4):87-98. (In Ukrainian).  CrossRef
  21. Mazzella N, Syakti AD, Molinet J, Gilewicz M, Doumenq P, Artaud J, Bertrand JC. Effects of crude oil on phospholipid fatty acid compositions of marine hydrocarbon degraders estimation of the bacteria membrane fluidity. Environ Res. 2005;97(5): 300-311. PubMed, CrossRef
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.