Tag Archives: sulfate-reducing bacteria

Glutathione level of Desulfovibrio desulfuricans ІМV K-6 under the influence of heavy metal salts

I. V. Kushkevych, S. O. Hnatush, H. V. Mutenko

Ivan Franko Lviv National University, Ukraine;
e-mail: Ivan_Kushkevych@ukr.net

Glutathione is the metal stress protector and changes of its level in the sulfate-reducing bacteria cells under the influence of heavy metal salts have not been studied yet. CdCl2, Pb(NO3)2, CuCl2, and ZnCl2 influence on the total glutathione level in cell-free extracts of sulfate-reducing bacteria Desulfovibrio desulfuricans ІМV K-6 was studied. The research has been carried out using Ellman, Lowry methods, statistical processing of the results. It was shown that the glutathione level depends on the heavy metal salts concentration in the medium. The total glutathione level was the highest under the influence of Pb(NO3)2. Other salts were also toxic to bacteria because glutathione level increased in bacterial cells after addition of these salts to the medium. On the basis of the results of our work the range of heavy metal salts influence on D. desulfuricans ІМV K-6 cells glutathione level has been formed for the first time: Pb(NO3)2 > CuCl2 > CdCl2 > ZnCl2.

Kinetic properties of adenosine triphosphate sulfurylase of intestinal sulfate-reducing bacteria

I. V. Kushkevych1,2, H. L. Antonyak3, M. Bartoš2

1Institute of Animal Biology, NAAS of Ukraine, Lviv;
2University of Veterinary and Pharmaceutical Sciences of Brno, Czech Republic;
3Ivan Franko National University of Lviv, Ukraine;
e-mail: ivan.kushkevych@gmail.com

The investigation of specific activity of ATP sulfurylase and kinetic properties of the enzyme in cell-free extracts of intestinal bacterial strains Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 is presented. The microbiological, biochemical, biophysical and statistical methods were used in the work. The optimal temperature (35 ºC) and pH 8.0-8.5 for enzyme reaction were determined. An analysis of kinetic pro­perties of ATP sulfurylase has been carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period, τ) and maximum veloci­ty of the ATP sulfurylase reaction (Vmax) have been defined. Michaelis constants (KmSulfate, KmATP, KmAPS, and KmPyrophosphate­) of the enzyme reaction were demonstrated for both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains.