Ukr.Biochem.J. 2014; Volume 86, Issue 6, Nov-Dec, pp. 129-138

doi: https://doi.org/10.15407/ubj86.06.129

Kinetic properties of adenosine triphosphate sulfurylase of intestinal sulfate-reducing bacteria

20.07.2015   Uncategorized   No comments

I. V. Kushkevych1,2, H. L. Antonyak3, M. Bartoš2

1Institute of Animal Biology, NAAS of Ukraine, Lviv;
2University of Veterinary and Pharmaceutical Sciences of Brno, Czech Republic;
3Ivan Franko National University of Lviv, Ukraine;
e-mail: ivan.kushkevych@gmail.com

The investigation of specific activity of ATP sulfurylase and kinetic properties of the enzyme in cell-free extracts of intestinal bacterial strains Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 is presented. The microbiological, biochemical, biophysical and statistical methods were used in the work. The optimal temperature (35 ºC) and pH 8.0-8.5 for enzyme reaction were determined. An analysis of kinetic pro­perties of ATP sulfurylase has been carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period, τ) and maximum veloci­ty of the ATP sulfurylase reaction (Vmax) have been defined. Michaelis constants (KmSulfate, KmATP, KmAPS, and KmPyrophosphate­) of the enzyme reaction were demonstrated for both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains.

Keywords: , , , , , , ,

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