Tag Archives: free radicals

Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver

B. І. Kotyk1, R. Ya. Iskra1, O. M. Slivinska1, N. M. Liubas1,
A. Z. Pylypets1, V. I. Lubenets2, V. I. Pryimych3

1Institute of Animal Biology, NAAS of Ukraine, Lviv;
2Lviv Polytechnic National University, Ukraine;
3Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine;
e-mail: kicyniabo@gmail.com

Received: 04 April 2020; Accepted: 25 June 2020

Ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. Structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. Therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to Cr(VI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (LHP) content in the rat liver caused by Cr(VI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (GSH) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular GSH in rat liver.

Influence of vermiculite particles on antioxidant properties of cultural medium of Bacillus subtilis IMV V-7023

I. O. Skorochod, I. K. Kurdish

Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: Kurdish@serv.imv.kiev.ua

It is shown that in the process of cultivation of Bacillus subtilis IMV V-7023 in the medium with vermiculite (1.5–5.0 g/l) one can observe the oppressing of some indexes of antioxidant properties of cultural medium of bacteria. In particular,  a decline of hydroxyl radical scavenging activity in the Fenton reaction by 2.8–11.6%, ability to inhibit formation of malondialdehyde – by 4.4–13.1% and inactivation of 2,2′-Diphenyl-1-picrylhydrazyl (DPPH·) radical – by 3.1–8.5% were observed. Thus oxidant activity increased substantially. Besides oppressing influence of particles of vermiculite on protector properties of the cultural medium of bacilli it is found out that with the increase of the content of dispersible material in the nutrient medium the reducing power of cultural medium of these bacteria increased.